Open Access Highly Accessed Editorial

MIQE précis: Practical implementation of minimum standard guidelines for fluorescence-based quantitative real-time PCR experiments

Stephen A Bustin*, Jean-François Beaulieu, Jim Huggett, Rolf Jaggi, Frederick SB Kibenge, Pål A Olsvik, Louis C Penning and Stefan Toegel

BMC Molecular Biology 2010, 11:74  doi:10.1186/1471-2199-11-74

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Best practice for qPCR experiments

Elizabeth Moylan   (2010-12-16 09:50)  BioMed Central

Readers of this Editorial may also find it useful to view the Science webinar on the topic entitled ‘The Future of qPCR: Best practices, Standardization, and the MIQE Guidelines’ (

Elizabeth C. Moylan PhD
Biology Editor, BMC series
BioMed Central

Competing interests



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