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MIQE précis: Practical implementation of minimum standard guidelines for fluorescence-based quantitative real-time PCR experiments

Stephen A Bustin1*, Jean-François Beaulieu2, Jim Huggett3, Rolf Jaggi4, Frederick SB Kibenge5, Pål A Olsvik6, Louis C Penning7 and Stefan Toegel8

Author Affiliations

1 Centre for Digestive Diseases, Barts and the London School of Medicine and Dentistry, Queen Mary University of London, 3rd Floor Alexandra Wing Royal London Hospital, London, UK

2 Canadian Institutes for Health Research Team on the Digestive Epithelium, Department of Anatomy and Cell Biology, Faculty of Medicine and Health Sciences, Université de Sherbrooke, Sherbrooke, QC, J1H 5N4, Canada

3 Molecular and Cell Biology, LGC, Queens Rd, Teddington, Middlesex TW11 OLY, UK

4 Department of Clinical Research, University of Bern, Murtenstrasse 35, CH-3010 Bern, Switzerland

5 OIE Reference Laboratory for ISA, Department of Pathology and Microbiology, Atlantic Veterinary College, University of Prince Edward Island, 550 University Avenue, Charlottetown, P.E.I., C1A 4P3, Canada

6 National Institute of Nutrition and Seafood Research, 5817 Bergen, Norway

7 Department of Clinical Sciences of Companion Animals, Faculty of Veterinary Medicine, Utrecht University, the Netherlands

8 Department of Pharmaceutical Technology and Biopharmaceutics, University of Vienna, Vienna, Austria

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BMC Molecular Biology 2010, 11:74  doi:10.1186/1471-2199-11-74

Published: 21 September 2010

Additional files

Additional file 1:

MIQE précis template for including as supplemental files in submitted manuscripts. *Strongly encouraged.

Format: XLS Size: 29KB Download file

This file can be viewed with: Microsoft Excel Viewer

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