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MIQE précis: Practical implementation of minimum standard guidelines for fluorescence-based quantitative real-time PCR experiments

Stephen A Bustin1*, Jean-François Beaulieu2, Jim Huggett3, Rolf Jaggi4, Frederick SB Kibenge5, Pål A Olsvik6, Louis C Penning7 and Stefan Toegel8

Author Affiliations

1 Centre for Digestive Diseases, Barts and the London School of Medicine and Dentistry, Queen Mary University of London, 3rd Floor Alexandra Wing Royal London Hospital, London, UK

2 Canadian Institutes for Health Research Team on the Digestive Epithelium, Department of Anatomy and Cell Biology, Faculty of Medicine and Health Sciences, Université de Sherbrooke, Sherbrooke, QC, J1H 5N4, Canada

3 Molecular and Cell Biology, LGC, Queens Rd, Teddington, Middlesex TW11 OLY, UK

4 Department of Clinical Research, University of Bern, Murtenstrasse 35, CH-3010 Bern, Switzerland

5 OIE Reference Laboratory for ISA, Department of Pathology and Microbiology, Atlantic Veterinary College, University of Prince Edward Island, 550 University Avenue, Charlottetown, P.E.I., C1A 4P3, Canada

6 National Institute of Nutrition and Seafood Research, 5817 Bergen, Norway

7 Department of Clinical Sciences of Companion Animals, Faculty of Veterinary Medicine, Utrecht University, the Netherlands

8 Department of Pharmaceutical Technology and Biopharmaceutics, University of Vienna, Vienna, Austria

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BMC Molecular Biology 2010, 11:74  doi:10.1186/1471-2199-11-74

Published: 21 September 2010


The conclusions of thousands of peer-reviewed publications rely on data obtained using fluorescence-based quantitative real-time PCR technology. However, the inadequate reporting of experimental detail, combined with the frequent use of flawed protocols is leading to the publication of papers that may not be technically appropriate. We take the view that this problem requires the delineation of a more transparent and comprehensive reporting policy from scientific journals. This editorial aims to provide practical guidance for the incorporation of absolute minimum standards encompassing the key assay parameters for accurate design, documentation and reporting of qPCR experiments (MIQE précis) and guidance on the publication of pure 'reference gene' articles.