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This article is part of the supplement: The International Conference on Intelligent Biology and Medicine (ICIBM) – Genomics

Open Access Research

Genomic sequence analysis and characterization of Sneathia amnii sp. nov

Michael D Harwich1, Myrna G Serrano12, Jennifer M Fettweis1, João MP Alves12, Mark A Reimers3, Vaginal Microbiome Consortium (additional members), Gregory A Buck12* and Kimberly K Jefferson1*

Author affiliations

1 Department of Microbiology and Immunology, Virginia Commonwealth University School of Medicine, 1101 E. Marshall Street - PO Box 980678, Richmond, VA 23298-0678, USA

2 Center for the Study of Biological Complexity, Virginia Commonwealth University, 1015 Floyd Avenue, PO Box 842030, Richmond, VA 23284-2030, USA

3 Department of Biostatistics, Virginia Commonwealth University School of Medicine, 830 East Main Street - PO Box 980032, Richmond, VA 23298-0032, USA

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Citation and License

BMC Genomics 2012, 13(Suppl 8):S4  doi:10.1186/1471-2164-13-S8-S4

Published: 17 December 2012

Abstract

Background

Bacteria of the genus Sneathia are emerging as potential pathogens of the female reproductive tract. Species of Sneathia, which were formerly grouped with Leptotrichia, can be part of the normal microbiota of the genitourinary tracts of men and women, but they are also associated with a variety of clinical conditions including bacterial vaginosis, preeclampsia, preterm labor, spontaneous abortion, post-partum bacteremia and other invasive infections. Sneathia species also exhibit a significant correlation with sexually transmitted diseases and cervical cancer. Because Sneathia species are fastidious and rarely cultured successfully in vitro; and the genomes of members of the genus had until now not been characterized, very little is known about the physiology or the virulence of these organisms.

Results

Here, we describe a novel species, Sneathia amnii sp. nov, which closely resembles bacteria previously designated "Leptotrichia amnionii". As part of the Vaginal Human Microbiome Project at VCU, a vaginal isolate of S. amnii sp. nov. was identified, successfully cultured and bacteriologically cloned. The biochemical characteristics and virulence properties of the organism were examined in vitro, and the genome of the organism was sequenced, annotated and analyzed. The analysis revealed a reduced circular genome of ~1.34 Mbp, containing ~1,282 protein-coding genes. Metabolic reconstruction of the bacterium reflected its biochemical phenotype, and several genes potentially associated with pathogenicity were identified.

Conclusions

Bacteria with complex growth requirements frequently remain poorly characterized and, as a consequence, their roles in health and disease are unclear. Elucidation of the physiology and identification of genes putatively involved in the metabolism and virulence of S. amnii may lead to a better understanding of the role of this potential pathogen in bacterial vaginosis, preterm birth, and other issues associated with vaginal and reproductive health.