The DFI calculation workflow. Rather than transforming whole datasets by normalization, each data point is compared to the other data points in the same dataset in a pair-wise fashion. The standard deviation of this ratio becomes a measure of the variability of a given gene among the multiple datasets being compared.
Ozer et al. BMC Genomics 2012 13(Suppl 8):S11 doi:10.1186/1471-2164-13-S8-S11