Screenshot of one of the novel cassette exon events. The diagram demonstrates reads supporting a cassette exon event that was not previously documented. This gene locates on the reverse strand. The genomic loci of the cassette exon, the 5' and 3' constitutive exon are chr1: 93073138-93073284, chr1: 93089733-93089891, and chr1: 93070864-93070959, respectively. A) Sequencing reads supporting the junction of the 5' and 3' constitutive exons, which indicates exon exclusion; B) Sequencing reads supporting the junction of the cassette exon and the 3' constitutive exon; C) Sequencing reads supporting the transcription of the cassette exon; D) Sequencing reads supporting the junction of the 5' constitutive exon and the cassette exon; E) UCSC gene annotation track; F) Alternative splicing annotation track; G) Human EST track; H) The cassette exon in UCSC gene annotations. No previous evidence of this cassette exon event was shown in the gene annotations, alternative splicing event annotations or the EST records.
Zhou et al. BMC Genomics 2012 13(Suppl 8):S10 doi:10.1186/1471-2164-13-S8-S10