Figure 3.

Screenshot of one of the novel cassette exon events. The diagram demonstrates reads supporting a cassette exon event that was not previously documented. This gene locates on the reverse strand. The genomic loci of the cassette exon, the 5' and 3' constitutive exon are chr1: 93073138-93073284, chr1: 93089733-93089891, and chr1: 93070864-93070959, respectively. A) Sequencing reads supporting the junction of the 5' and 3' constitutive exons, which indicates exon exclusion; B) Sequencing reads supporting the junction of the cassette exon and the 3' constitutive exon; C) Sequencing reads supporting the transcription of the cassette exon; D) Sequencing reads supporting the junction of the 5' constitutive exon and the cassette exon; E) UCSC gene annotation track; F) Alternative splicing annotation track; G) Human EST track; H) The cassette exon in UCSC gene annotations. No previous evidence of this cassette exon event was shown in the gene annotations, alternative splicing event annotations or the EST records.