The basic steps in searching for differential expression genes. EST library selection involves selection of suitable EST clone libraries, EST to gene assignment, counting the results, remove tissue categories with low counts, statistical analysis with CMH and the narrow-down of differentially expressed genes (DEGs). The narrow-down procedures includes cross referencing with public microarray data, annotating membrane and secretory proteins, analyzing with String network, and for a few selected genes, validate the expression in different tissues by RT-qPCR.
Wu et al. BMC Genomics 2012 13(Suppl 7):S12 doi:10.1186/1471-2164-13-S7-S12