Gene expression patterns in TLR-stimulated dendritic cells and identification of a TF pair synergistically upregulating target promoters. (A) Hierarchical clustering of gene expression in DCs upon TLR-stimulation. A heatmap of gene expression is shown with clusters indicated by the colored banner besides the heatmap. (B) The Nos2 promoter was synergistically controlled by RelA or MyD88 with C/EBPα. Schematic representation of the Nos2 promoter structure is shown (red box, NF-κB sites; blue box, C/EBPα sites). The Nos2 promoter was cloned upstream of a luciferase encoding gene. The resulting plasmid, where luciferase is under the control of the Nos2 promoter, was transfected into HEK 293 cells with RelA or MyD88 and C/EBPα. After 24 hours, luciferase activity was measured as described in Methods. Error bars represent standard deviations of duplicate experiments. The data shown is a representative of three independent experiments with essentially identical results.
Vandenbon et al. BMC Genomics 2012 13(Suppl 7):S11 doi:10.1186/1471-2164-13-S7-S11