Table 5

Upregulation of key target enzymes when H2 is produced under mixotrophic conditions, highlighted by AlgaGEM

Step reaction

Regulation (up/down)

Reference*


Cyclic electron flow (PSI)

-

[48]

Linear electron flow (PSII)

+

[48]

TCA cycle/mitochondria

-

[48]

Fe-Hydrogenases

+

[29,31,35,49-52]

Ferredoxin-NADP+ reductase (FNR)

+

[53]

Glyceraldehyde 3-P-dehydrogenase

+

[54-57]

Pyruvate ferredoxin oxireductase

+

[58-60]

Calvin Cycle/CO2 assimilation

-

[44]

Acetate assimilation

+

[44]

Gluconeogenesis (cytosolic)

+

Not found

Pentose phosphate pathway (cytosolic)

-

Not found

Pentose phosphate pathway (plastidic)

+

Not found

Beta oxidation/glyoxylate cycle

-

Not found

glutamate synthase (ferredoxin);GSGOGAT

+

Not found

glutamine synthetase; GS/GOGAT

-

Not found


*Results supported by evidences found in the literature. Not found: model highlights new targets in different pathways to improve H2 that need further experimental investigation for model validation.

Gomes de Oliveira Dal’Molin et al. BMC Genomics 2011 12(Suppl 4):S5   doi:10.1186/1471-2164-12-S4-S5

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