Open Access Research article

Increasing the source/sink ratio in Vitis vinifera (cv Sangiovese) induces extensive transcriptome reprogramming and modifies berry ripening

Chiara Pastore1, Sara Zenoni2, Giovanni Battista Tornielli2, Gianluca Allegro1, Silvia Dal Santo2, Gabriele Valentini1, Cesare Intrieri1, Mario Pezzotti2* and Ilaria Filippetti1

Author Affiliations

1 Department of Fruit Tree and Woody Plant Science, University of Bologna, Viale Fanin, 46, 40126, Bologna, Italy

2 Department of Biotechnology, University of Verona, Strada Le Grazie 15, 37134, Verona, Italy

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BMC Genomics 2011, 12:631  doi:10.1186/1471-2164-12-631

Published: 23 December 2011

Additional files

Additional file 1:

Differentially expressed genes along berry development displaying a two-fold or greater change in transcript abundance between EV and BV or EV and H, in CT and C treatments. For each gene the annotation, the EV/BV and the H/BV Fold-change (FC) are indicated. Data obtained for CT and C are listed in two separate worksheets.

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Additional file 2:

Differentially expressed genes along berry development displaying a five-fold or greater change in transcript abundance between EV and BV or EV and H, in CT and C treatments. For each gene the annotation, the gene ontology, the EV/BV and the H/BV Fold-change (FC), the expression profile and the expression behavior in the other treatment are reported. Data obtained for CT and C are listed in two separate worksheets.

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Additional file 3:

Real time RT-PCR validation of six selected genes. Expression profiles measured by real time RT-PCR are determined by calculating the relative expression ratio value for each stage relative to the BV stage. Real time RT-PCR data are reported as means ± SE of three biological replicates, obtained by using two reference genes. An actin beta/gamma 1 (VIT_12s0178g00200) and an elongation factor 1 (VIT_06s0004g03220) were used as control genes. For each gene the expression profile obtained by microarray analysis is shown on the right side. A-B: Vacuolar invertase 1, GIN 1 (VIT_16s0022g00670); C-D: PAL [Vitis vinifera] (VIT_16s0039g01120); E-F: Flavonol synthase (VIT_18s0001g03430); G-H: β-Fructosidase -invertase (VIT_00s2527g00010); I-J: Flavonoid 3’5’-hydroxylase (VIT_06s0009g02910); K-L: UDP-glucose:flavonoid glucosyltransferase (VIT_04s0023g01290).

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Additional file 4:

Differentially expressed genes along berry development displaying a similar expression profile between C and CT. For each gene, the annotation, the gene ontology, the profile and the CT/C Fold-change (FC) ratio at EV and at H are reported.

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Additional file 5:

Differentially expressed genes along CT berry development encoding ABC and MATE transporters. Analysis was performed by querying the protein sequence of the probe consensus against the NCBI databases using BLASTX. For each gene the correspondent Arabidopsis thaliana homologue, the NCBI reference sequence and E-value are specified.

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Additional file 6:

Differentially expressed genes displaying a two-fold or greater change in transcript abundance between C and CT at EV and H time points. For each gene the description and the CT/C Fold-change (FC) are indicated. Data obtained for EV and H are listed in two separate worksheets.

Format: XLS Size: 467KB Download file

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Additional file 7:

Differentially expressed genes displaying a five-fold or greater change in transcript abundance between C and CT at EV and /or H. For each gene the CT/C Fold-change (FC) at EV and /or H, the description, the gene ontology, the presence of the gene among genes identified by cluster analysis as CT specific, C specific, common to C and CT with a different expression profile and common to C and CT with the same expression profile, are reported..

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Additional file 8:

List of the primers used for real time RT-PCR validation experiment.

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