Table 2

Summary of shRNA pool deconvolution by next-generation sequencing

Statistic

Even

4x

16x

64x

2-20x


Reads passed filter

22407757

24596943

24793754

24304519

25684516

Mapped reads

22296083

24504006

24687271

24235485

25605983

Unmapped reads

111674

94937

106483

69034

78533

Unmapped reads %

0.5

0.39

0.43

0.28

0.31

1st quartile

40

34

26

35

21

Median

153

149

140

162

107

Mean

315

352.4

351.6

350.7

370.2

3rd quartile

375

408

404

417

385

Max

17960

19360

21750

20000

29460

Uncounted

9784

10748

10045

10983

10739


Ketela et al. BMC Genomics 2011 12:213   doi:10.1186/1471-2164-12-213

Open Data