Oligonucleotide design strategy for Combimatrix custom oligonucleotide array. Probes were designed complementary to the predicted mature miRNA (green line) and miRNA* (thick black line) sequences. Additional probes were designed to the loop region (thin black line) as well as probes shifted 5 nucleotides (red lines) and 10 nucleotides (blue lines) with respect to the miRNA and miRNA* sequences.
Mica et al. BMC Genomics 2010 11:109 doi:10.1186/1471-2164-11-109