Research article
High throughput approaches reveal splicing of primary microRNA transcripts and tissue specific expression of mature microRNAs in Vitis vinifera
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* Corresponding author: David S Horner david.horner@unimi.it
- † Equal contributors
1 Dipartimento di Scienze Biomolecolari e Biotecnologie, Università degli Studi di Milano, Milano, Italy
2 Scuola Superiore Sant'Anna, Pisa, Italy
3 Dipartimento di Biotecnologie, Università degli Studi di Verona, Verona, Italy
4 Dipartimento di Scienze Tecnologie e Mercati della Vite e Vino, Università degli Studi di Verona, Verona, Italy
5 Dipartimento di Scienze Agrarie ed Ambientali, Università degli Studi di Udine, Udine, Italy
6 CRIBI Biotechnology Centre, Dipartimento di Biologia, Università degli Studi di Padova, Padova, Italy
7 Istituto di Genomica Applicata, Udine, Italy
8 Istituto Tecnologie Biomediche, Consiglio Nazionale delle Ricerche, Bari, Italy
9 Dipartimento di Biochimica e Biologia Molecolare "E. Quagliariello", Università di Bari, Bari, Italy
BMC Genomics 2009, 10:558 doi:10.1186/1471-2164-10-558
Published: 25 November 2009Additional files
Additional file 1:
Expression data for Vitis vinifera miRNAs. Supplemental Table S1: Frequencies of mapped small RNA reads by size and locus. Supplemental Figure S2: Log expression levels of all predicted miRNAs in all tissues as detected by oligonucleotide array. Supplemental Figure S3: Detailed alignments and frequencies of all small RNA reads mapping to miRNA precursors. Supplemental Table S4: transcript data for 25 Vitis vinifera miRNAs. Supplemental Figure S5: Splice junction read coverage for Vvi-miR394b, Vvi-miR162 and Vvi-miR168.
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Additional file 2:
Oligonucleotide Array probes for analysis of Vitis vinifera miRNA expression. Supplemental Table S6: All oligonucleotide array probe sequences.
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