Neighbour-joining phylogenetic tree of the functional genomic repertoire of murine, human and bovine TRBV genes. (A) Analysis of the coding region nucleotide sequences of functional TRBV genes following complete deletion to remove gaps in the alignment. The final dataset included 281 positions. The sequence of HTRAV1-1 has been included as an outgroup. The six phylogenetic groups (A-F) have been indicated and the percentage bootstrap interior branch test value (PB) based on a 1000 replications is shown for each of the groups. To reduce the size of the tree for presentation, where 2 TRBV genes have identical sequence only 1 has been included in the analysis. Therefore, bovine 6x (identical to 6t), 6an (6p), 6u (6y), 6ag (6l), 6ak (6h), 6v (6z), 9ae (9f), 9ak (9b), 9ac(9k), 9s (9v), 9aj (9d), 19d (19e) and 21m (21p) have been excluded, as has human TRBV6-3 which is identical to TRBV6-2. H = human, M = murine, B = bovine. (B) Summary of the distribution of functional TRBV genes amongst the 6 phylogenetic groups in humans, mice and Btau_3.1.
Connelley et al. BMC Genomics 2009 10:192 doi:10.1186/1471-2164-10-192