WC1 Domain 1 sequence. (A) WC1 Domain 1 deduced amino acid sequences were aligned with ClustalW2 using the default parameters and visualized with JalView. Analysis includes all non-redundant genomic sequences and all non-redundant cDNA sequences. The archetypal WC1 sequence (WC1.1, GenBank accession number X63723) and swine WC1 sequence (swppWC1, GenBank accession number CAA67710) were included in the analysis for comparison. WC1-6 sequence is partial due to poor genomic sequence integrity. (B) Phylogenetic tree generated using WC1 Domain 1 deduced amino acid sequences and the Neighbor-Joining method . Archetypal WC1 (WC1.1) and swine WC1 (swppWC1) Domain 1 sequences (accession numbers above) were included for comparison and archetypal WC1 Domain 2 (WC1.1D2) sequence was included to root the tree. The optimal tree with the sum of branch length = 2.02452921 is shown with bootstrap values (based on 1000 replicates) reported next to the branches. Positions containing alignment gaps were eliminated only in pairwise sequence comparisons for a total of 112 positions in the final dataset.
Herzig and Baldwin BMC Genomics 2009 10:191 doi:10.1186/1471-2164-10-191