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Open Access Highly Accessed Research article

Transcriptional reprogramming of gene expression in bovine somatic cell chromatin transfer embryos

Nelida Rodriguez-Osorio14, Zhongde Wang2*, Poothappillai Kasinathan2, Grier P Page3, James M Robl2 and Erdogan Memili1*

Author affiliations

1 Department of Animal and Dairy Sciences, Mississippi State University, Starkville, MS, USA

2 Hematech Inc, Sioux Falls, SD, USA

3 University of Alabama-Birmingham, Birmingham AL, USA

4 Grupo CENTAURO, Universidad de Antioquia, Medellín, Antioquía, Colombia

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Citation and License

BMC Genomics 2009, 10:190  doi:10.1186/1471-2164-10-190

Published: 24 April 2009

Abstract

Background

Successful reprogramming of a somatic genome to produce a healthy clone by somatic cells nuclear transfer (SCNT) is a rare event and the mechanisms involved in this process are poorly defined. When serial or successive rounds of cloning are performed, blastocyst and full term development rates decline even further with the increasing rounds of cloning. Identifying the "cumulative errors" could reveal the epigenetic reprogramming blocks in animal cloning.

Results

Bovine clones from up to four generations of successive cloning were produced by chromatin transfer (CT). Using Affymetrix bovine microarrays we determined that the transcriptomes of blastocysts derived from the first and the fourth rounds of cloning (CT1 and CT4 respectively) have undergone an extensive reprogramming and were more similar to blastocysts derived from in vitro fertilization (IVF) than to the donor cells used for the first and the fourth rounds of chromatin transfer (DC1 and DC4 respectively). However a set of transcripts in the cloned embryos showed a misregulated pattern when compared to IVF embryos. Among the genes consistently upregulated in both CT groups compared to the IVF embryos were genes involved in regulation of cytoskeleton and cell shape. Among the genes consistently upregulated in IVF embryos compared to both CT groups were genes involved in chromatin remodelling and stress coping.

Conclusion

The present study provides a data set that could contribute in our understanding of epigenetic errors in somatic cell chromatin transfer. Identifying "cumulative errors" after serial cloning could reveal some of the epigenetic reprogramming blocks shedding light on the reprogramming process, important for both basic and applied research.