Table 2

The oligonucleotides used for quantitative PCR to measure relative transcript abundance of ancient bovine PAGs during pregnancy.

PAG

Sequence

Primer location (bp)

Amplicon size (bp)


BoPAG-2

F: 5'-GTAGGCTCGCCTATCACCATCTTC-3'

360

289

R: 5'-CCTCTGGCTTGTTTGTGTTCAAGTAG-3'

649

BoPAG-8

F: 5'-CCTATCCTGAATGACGAGCAA-3'

886

128

R: 5'-CCTTTCAGAAAACCTCTGGATG-3'

1014

BoPAG-10

F: 5'-TTGAGCAGTCAGAAAGAGAACG-3'

631

136

R: 5'-TTCATGGAGATGCTGTCTATGTTT-3'

767

BoPAG-11

F: 5'-CGGTTCCGAGTACATGGTTT-3'

885

114

R: 5'-AGAAATCTTTTGGATGTAGGCTTC-3'

999

BoPAG-12

F: 5'-ACACACCAGCCTATTAGCATCTCC-3'

361

266

R: 5'-CGGCTGGCATGTGTTCAAGTAG-3'

627

YHWAG

F: 5'-AGCACATGCAGCCCACTC-3'

488

120

R: 5'-TCGTCGAAGGCGGTCTTG-3'

608


Telugu et al. BMC Genomics 2009 10:185   doi:10.1186/1471-2164-10-185

Open Data