Table 6

Effect of masking spermatozoa ITGB5 protein during fertilization.

Total

Unfertilized

Fertilized

Not


Groups

Oocytes

(N)

Oocytes

N (%)

Normally

N (%)

Determined

N (%)


Anti-ITGB5*

0

330

45 (13)a

242 (74)a

43 (13)a

(μg/ml)

5

313

106 (34)b

190 (60)ab

17 (5)a

20

293

116 (40)b

137 (47)b

40 (14)a

Anti-BIT*

(μg/ml)

20

263

45 (17)a

190 (72)a

28 (11)a


*Bovine spermatozoa were exposed to antibodies (anti-ITGB5 and anti-BIT) for 2 h before being using to fertilize the oocytes.

"Fertilized oocytes" refers to oocytes observed with at least one pronucleus, while "Unfertilized oocytes" refers to those without any pronuclei, but containing a Germinal Vesicle or Metaphase-1 or -2 structures (with only one extruded polar body).

(%) Percentage expressed on total oocytes.

a, b Values with different superscripts within the same columns are significantly different (Student's test; p < 0.05).

Feugang et al. BMC Genomics 2009 10:176   doi:10.1186/1471-2164-10-176

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