Email updates

Keep up to date with the latest news and content from BMC Bioinformatics and BioMed Central.

This article is part of the supplement: Proceedings of the Fifth Annual MCBIOS Conference. Systems Biology: Bridging the Omics

Open Access Proceedings

Wanted: unique names for unique atom positions. PDB-wide analysis of diastereotopic atom names of small molecules containing diphosphate

Christopher A Bottoms and Dong Xu*

Author Affiliations

Department of Computer Science and Christopher S. Bond Life Sciences Center, University of Missouri, Columbia, MO 65211, USA

For all author emails, please log on.

BMC Bioinformatics 2008, 9(Suppl 9):S16  doi:10.1186/1471-2105-9-S9-S16


The electronic version of this article is the complete one and can be found online at: http://www.biomedcentral.com/1471-2105/9/S9/S16


Published:12 August 2008

© 2008 Bottoms and Xu; licensee BioMed Central Ltd.

This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background

Biological chemistry is very stereospecific. Nonetheless, the diastereotopic oxygen atoms of diphosphate-containing molecules in the Protein Data Bank (PDB) are often given names that do not uniquely distinguish them from each other due to the lack of standardization. This issue has largely not been addressed by the protein structure community.

Results

Of 472 diastereotopic atom pairs studied from the PDB, 118 were found to have names that are not uniquely assigned. Among the molecules identified with these inconsistencies were many cofactors of enzymatic processes such as mononucleotides (e.g. ADP, ATP, GTP), dinucleotide cofactors (e.g. FAD, NAD), and coenzyme A. There were no overall trends in naming conventions, though ligand-specific trends were prominent.

Conclusion

The lack of standardized naming conventions for diastereotopic atoms of small molecules has left the ad hoc names assigned to many of these atoms non-unique, which may create problems in data-mining of the PDB. We suggest a naming convention to resolve this issue. The in-house software used in this study is available upon request.

A version of the software used for the analyses described in this paper is available at our web site: http://digbio.missouri.edu/ddan/DDAN.htm webcite.

Background

Often accompanying the macromolecules deposited in the Protein Data Bank (PDB) [1] are smaller molecules of biological importance. Some of these are energy-carrying cofactors, such as ATP, coenzyme A, and nicotinamide-adenine dinucleotide (NAD). Some analogs of these molecules are either drugs or can be used in drug design [2,3].

Like other biologically relevant molecules, many of these small molecules contain chiral or prochiral centers. An atom is a chiral center if four different chemical groups are attached to it. A chiral configuration can be designated R or S, depending on the arrangement of the attached groups (Figure 1). If, however, two of these groups are identical, then the center atom is prochiral, meaning that it would become chiral if either of the identical groups were substituted for a unique group. These two groups are called diastereotopic, i.e., if either were replaced with a unique group, the molecule would become one or another diastereomer. Within a pair of diastereotopic atoms, one is designated pro-R and the other pro-S, indicating the configuration of the chiral atom would result from replacing the diastereotopic atom with a group that has higher priority than the other groups. Many ligands contain diphosphate groups that contain at least one prochiral phosphorus atom (Figure 2).

thumbnailFigure 1. S and R configurations for chiral centers. (a) S configuration and (b) R configuration, for atoms A, B, C, and D when they have the highest, second, third, and lowest priorities, respectively. Notice that when the three highest priority groups (A, B, and C) are facing the viewer, they have a counter-clockwise arrangement in the S configuration and a clockwise arrangement in the R configuration.

thumbnailFigure 2. NAD molecules from X-ray crystal structure 2OHX. For comparison purposes, one molecule is superimposed on the other and then offset slightly. The atom names are similarly offset for the diastereotopic oxygen atoms of the adenine-side phosphate group. Note the differences in names for the pro-R and pro-S atoms. Both molecules of NAD shown are from an alcohol dehydrogenase structure [PDB:2OHX][16]. Following the CIP-algorithm, since all four oxygen atoms have the same atomic number, their priority is determined by subsequent bonded groups. The O3 oxygen atom is bonded to the next phosphorus atom and the O5' oxygen atom is bonded to the preceding C5' carbon atom, while the remaining two oxygen atoms are unbonded except to the original phosphorus atom. Therefore, the O3 oxygen atom has the highest priority, the O5' oxygen atom has the second highest priority, and the remaining two oxygen atoms tie for the lowest priority. The pro-S atom is the one that, if it were replaced with an atom of highest priority, would make the phosphorus atom chiral with an S configuration. Both molecules are drawn using red for oxygen, blue for nitrogen, and orange for phosphorus. One is drawn using light blue for carbon and the other is drawn using white for carbon.

The pro-S and pro-R oxygen atoms of nucleic acid strands are named "OP1" and "OP2", respectively [4]. Many enzymes treat the pro-R and pro-S oxygen atoms of DNA and RNA differently [5]. These diastereotopic oxygen atoms are also treated differently in RNA-intron splicing [6,7]. Small diphosphate-containing molecules also participate in enzymatic reactions in which the distinction between diastereotopic atoms or groups is important [5,8,9]. Unfortunately, many of these diastereotopic atoms do not have standardized names, an issue that has not been investigated to our knowledge. Consistent naming of diastereotopic atoms is needful when performing all-atom superpositioning or all-atom root mean square deviation (RMSD) calculation [10]. It is also needful for data mining in the PDB, e.g., structure-based virtual screening for drug candidates [11,12]. In this paper, we will conduct a systematic PDB-wide analysis on the diastereotopic atom names of small molecules containing diphosphate.

Results

Inconsistencies in PDB files

There were 4167 PDB files containing a total of 295 distinct ligands having prochiral centers that met our strict criteria. Over half of these ligands (175) had two prochiral phosphate centers that were adjacent to carbon, and one had three (OXT from [PDB:2JI7] [13]), for a total of 472 distinct prochiral centers adjacent to carbon. For example, NAD contains two because it has a diphosphate sandwiched between two ribose moieties. Each distinct prochiral center contains a pair of disastereotopic atoms. We analyzed the names of the atoms at each prochiral center. Of these distinct centers, 354 had a single naming convention but 241 of these also only occurred in a single PDB file. There were 118 distinct prochirality centers that had more than one naming convention.

We defined a case of swapped names to occur when all of the following were true between two molecules with the same type of prochiral center: (1) the highest and second highest priority names were consistent, (2) the pro-R atom of one prochiral center had the same name as the pro-S atom of a second center, and (3) the pro-S atom of the first center had the same name as the pro-R atom of the second center (Figure 2). 117 of the 118 centers had swapped naming conventions as defined above. The remaining center, which had two naming conventions, actually had a naming error. Nine of the 117 centers with swapped names had additional naming conventions. In every case, we found that the extra naming conventions were caused by errors rather than mere inconsistencies. For example, in a structure of a surfactin synthetase-activating enzyme [PDB:1QR0] [14], the diastereotopic atoms attached to phosphorus atom P1A are labeled "O5A" and "O4A" instead of the names "O2A" and "O1A" defined in the Chemical Component Dictionary http://deposit.rcsb.org/het_dictionary.txt webcite from the PDB. In a similar manner, the diastereotopic atoms attached to P2A are named "O2A" and "O1A", instead of the names "O5A" and "O4A" defined in the dictionary file. In another example, in a structure of E. coli carbamoyl phosphate synthetase [PDB:1CE8] [15] the O5' oxygen atom is mislabeled as O4' for 8 different ADP molecules. Interestingly, in four of these molecules, the pro-S and pro-R atoms are labeled "O1A" and "O2A", respectively, while in the other four molecules they are labeled "O2A" and "O1A", respectively.

In Table 1, we present statistics for sample cases in which there were at least two nonredundant examples of each naming convention. For additional selected examples, see Supplement Table 1 in Additional File 1. For our full results, including cases that had no inconsistencies, see Supplemental Table 2 in Additional File 2 (explanation in Additional file 3). All results, including those resulting from errors, are included in Supplemental Table 2. However, we emphasize that the bulk of the results are due to inconsistencies, not errors.

Additional file 1. Supplemental Table 1. Contains Table 1 from this document with about four additional pages of examples of naming convention statistics for selected ligands.

Format: DOC Size: 383KB Download file

This file can be viewed with: Microsoft Word ViewerOpen Data

Additional file 2. Supplemental Table 2. Contains all of the calculated results, including those for prochiral centers that appear only once in the PDB.

Format: XLS Size: 9.6MB Download file

This file can be viewed with: Microsoft Excel ViewerOpen Data

Additional file 3. Explanation of Supplemental Table 2. Contains an explanation of the columns in Supplemental Table 2.

Format: DOC Size: 30KB Download file

This file can be viewed with: Microsoft Word ViewerOpen Data

Table 1. Naming convention statistics for selected ligands

Examples of naming inconsistencies

Most of the atom naming inconsistencies mentioned in this paper relate to differences found between different files. However, there are a few cases in which naming inconsistencies can be found within a single file. One example is an X-ray crystal structure of alcohol dehydrogenase [PDB:2OHX] [16]. This structure contains two NAD molecules (see Figure 2). The prochiral center around phosphorus atom PN has consistent naming between the two molecules, however the prochiral center around phosphorus atom PA does not. In one case the pro-S and pro-R atoms are named "O1A" and "O2A", respectively, and in the other case, the names are "O2A" and "O1A", respectively.

Another example is an NMR structure of bovine acyl-coenzyme A binding protein (Figure 3) [PDB:1NVL]. This structure contained 20 NMR models, in which one phosphorus prochiral center was consistently named and the other was not. For the P1A center, models 1, 2, 5 and 18 have pro-S and pro-R atoms named "O1A" and "O2A", while the remaining 15 models have them named "O2A" and "O1A", respectively. Meanwhile, the pro-S and pro-R atoms at the P2A center are consistently named "O5A" and "O4A", respectively.

thumbnailFigure 3. Diphosphate of Coenzyme A from NMR structure 1NVL. The diphosphate region of coenzyme A of two models from 1NVL is shown. The diphosphate region of model 4 (light coloring) is superimposed on the same diphosphate region of model 2 (standard coloring). The diastereotopic names of prochiral center P2A have consistent names (O4A and O5A), but the pro-S and pro-R names for prochiral center P1A are not (O1A and O2A, respectively, for model 2, and O2A and O1A, respectively, for model 4).

Discussion

The inconsistent naming of atoms discussed in our paper is due largely to a lack of standardized names, not due to errors on the part of crystallographers or NMR researchers. There can be no errors where there are no rules.

A study of NAD(P) molecules by Carugo and Argos ignored the diastereotopic oxygen atoms for purposes of superimposing molecules because of naming inconsistencies [17]. Despite their use of atom-specific names for other atoms in the molecules, they only generally referred to diastereotopic oxygen atoms as "terminal oxygen atoms". That was eleven years ago and only involved a study of 32 protein structures. This was long before the recent remediation project of the PDB [18]. This project has done well to bring molecular and atomic naming conventions for PDB files into conformity with standards established by the International Union of Pure and Applied Chemistry (IUPAC) and the International Union of Biochemistry and Molecular Biology (IUBMB). However, IUPAC and IUBMB do not have standards for most diastereotopic atoms of small molecules.

There were no obvious overall trends in naming conventions with respect to the pro-R and pro-S atoms. This is likely due to the lack of naming standardization. However, trends are commonly seen among specific ligands (Table 1). One interesting observation is that the P prochiral center of FAD is highly biased in its naming convention (87% for one convention); however, the second center, PA has little bias (54% for one convention). Another observation is that NAD-like ligands tend to have naming conventions such that similar names (e.g. O1A and O1N) are seen on the same "side" of the molecule.

We suggest a general rule that names for pro-S atoms come alphanumerically before names for pro-R atoms. This is similar to the standard of using "OP1" for pro-S and "OP2" for pro-R in nucleic acids. The data indicates that there is no strong bias for this nor for its opposite convention among diphosphate containing ligands.

Regardless of what rules may become adopted, it is important to know to which atom a particular name refers. Establishing standard names and topologies that take prochirality into consideration will result in less confusion and more accuracy in studies involving small molecules. Until standards are adopted, individuals mining the data need to do their own standardization of the names. This naming can be enforced upfront, prior to the official release of data, or it can be enforced by individuals mining the data.

Conclusion

Current naming conventions do not completely map unique names to unique diastereotopic atoms, resulting in possible confusion or error, or at least the need for researchers to impose their own naming standardization. We herein describe many cases of naming inconsistencies for small molecules containing diphosphate moieties. A future study will assess naming conventions of all atoms in the PDB, addressing more general issues of chirality and prochirality. The in-house software used in this study is available upon request.

Methods

Selection of small molecules for analysis

PDB files were selected from the January 7, 2008 "snapshot" of the Protein Data Bank. The search feature of the Protein Data Bank website http://www.pdb.org/pdb/search/advSearch.do webcite was used to select PDB codes for files containing ligands that had substructures matching the SMILES pattern "C~O~P(~O)(~O)~O~P(~O)(~O)~O". Here, "C" represents a carbon atom, "~" represents any bond, "O" represents oxygen, "P" represents phosphorus, and the parentheses indicate that the oxygen atoms inside them are bonded to the preceding phosphorus atom in the list, not to subsequent atoms in the list. This matches any ligand containing a (PO4)2 moiety, such as NAD, ATP, and Coenzyme A, resulting in a list of 4435 PDB codes.

Since the PDB files corresponding to these codes also included other ligands not meeting our criteria, we analyzed each of the small molecules within each PDB file and selected each one that met the following criteria: (1) It did not have the same residue name as an amino acid or nucleic acid, including names mapped to standard residue names via the "MODRES" record. (2) It had an entry in the Chemical Component Dictionary http://deposit.rcsb.org/het_dictionary.txt webcite from the PDB. (3) It had complete coordinates for the non-hydrogen atoms specified in the Chemical Component Dictionary. And (4), it had a diphosphate group attached to carbon, with the diphosphate group consisting of two phosphorus atoms, each covalently bonded to four oxygen atoms. We chose to analyze the prochiral phosphate centers adjacent to carbon atoms because of their abundance and because it allowed a simple and direct application of the CIP algorithm.

Atoms were considered to be covalently bonded if the distance between their centers was less than the sum of their covalent radii plus a cushion of 0.4 Å, following the custom of the Cambridge Structural Database (CSD) [19]. Covalent radii were obtained from the CSD website http://www.ccdc.cam.ac.uk/products/csd/radii/ webcite.

Also excluded were molecules that had alternate conformations that shared the same residue number. This guaranteed that any modeled alternate conformations would contain complete molecules. Those files containing diphosphates were further checked for phosphorus atoms having a prochiral configuration (see Determination of Prochiral Centers below). For those that did, the names of all four atoms attached to the prochiral center were recorded along with their relative stereochemical positions. Of the 4435 files originally selected, 4184 were found to have at least one ligand with a prochiral phosphate atom.

Determination of prochiral centers

The CIP algorithm [20,21] for assigning priorities to atoms within a molecule was implemented using in-house software. CIP priorities were calculated for all four atoms connected to a phosphorus atom. Following the CIP-algorithm, the oxygen atom attached to two phosphorus atoms always had the highest priority and the oxygen atom attached to carbon always had the second highest priority. The two remaining oxygen atoms were not bonded to any other atom besides the phosphorus atom.

If each atom had a distinct priority, then the phosphorus is chiral and the determinant algorithm of Cieplak and Wisniewski[22] could be used to calculate whether the configuration is R or S as shown in Equation (1):

<a onClick="popup('http://www.biomedcentral.com/1471-2105/9/S9/S16/mathml/M1','MathML',630,470);return false;" target="_blank" href="http://www.biomedcentral.com/1471-2105/9/S9/S16/mathml/M1">View MathML</a>

(1)

XN, YN, and ZN are the x, y, and z components of the coordinates for group N. The subscripted letters A, B, C, and D represent the highest, second highest, third highest, and lowest priority atoms, respectively (see Figure 1). m is the result of calculating the determinant. It is negative for the R configuration and positive for the S configuration. If it is evaluated to be zero, then the atoms are all in the same plane [22], which should never be the case for tetrahedrally arranged molecules such as phosphates. For understanding the mathematics behind this equation and how it captures the handedness of four three-dimensional coordinates, we refer the reader to the work of Cieplak and Wisniewski [22].

If two of the atoms attached to the phosphorus atom have identical priorities, then they are diastereotopic and the phosphorus is prochiral. In the case of diphosphate-containing molecules, the diastereotopic atoms are only bonded to phosphorus and therefore have the lowest priority (see Figure 2). We will call the atoms attached to the phosphorus atom A, B, C, and C', where A and B have the highest and second highest priority, respectively, while C and C' tie for the lowest priority. In this case, Equation (1) can be adapted to determine whether C is the pro-S or pro-R atom and, concomitantly, whether C' is the pro-R or pro-S atom. By definition, a diastereotopic atom being pro-S (or pro-R) means that, if it were replaced by a group with higher priority than any other substituent, then the prochiral center would become chiral with an S (or R) configuration. Therefore, we treat C as if it had the highest priority and then calculate the resulting configuration. If the calculated configuration is S, then C is pro-S; if it is R, then C is pro-R. To do this computationally, we artificially raise the priority of C to be the highest (i.e. higher than A) changing Equation (1) to the following:

<a onClick="popup('http://www.biomedcentral.com/1471-2105/9/S9/S16/mathml/M2','MathML',630,470);return false;" target="_blank" href="http://www.biomedcentral.com/1471-2105/9/S9/S16/mathml/M2">View MathML</a>

(2)

If m is positive, then C is the pro-S atom and, concomitantly, C' is the pro-R atom (Figure 2). If m is negative, then C is the pro-R atom and C' is the pro-S atom.

Third-party software used

COOT [23] was used for visualizing PDB files, which was especially useful during the development of our software. As needed, the SSM [24] module of COOT was also used for superposition of molecules. Pymol was used for viewing NMR models as well as generating depictions of molecular structures for figures [25].

Competing interests

The authors declare that they have no competing interests.

Authors' contributions

CAB participated in the design of the study, developed the in-house software, carried out the atom name analysis, and drafted the manuscript. DX participated in the design and coordination of the study, and helped draft the manuscript. Both authors read and approved the final manuscript.

Acknowledgements

CAB was supported by NIH Grant Number 2-T15-LM07089-16 from the National Library of Medicine. DX was supported by an NIH Grant (1R21GM078601-01).

This article has been published as part of BMC Bioinformatics Volume 9 Supplement 9, 2008: Proceedings of the Fifth Annual MCBIOS Conference. Systems Biology: Bridging the Omics. The full contents of the supplement are available online at http://www.biomedcentral.com/1471-2105/9?issue=S9

References

  1. Berman HM, Westbrook J, Feng Z, Gilliland G, Bhat TN, Weissig H, Shindyalov IN, Bourne PE: The Protein Data Bank. [http://www.rcsb.org/] webcite

    Nucleic Acids Res 2000, 28(1):235-242. PubMed Abstract | Publisher Full Text | PubMed Central Full Text OpenURL

  2. Faig M, Bianchet MA, Winski S, Hargreaves R, Moody CJ, Hudnott AR, Ross D, Amzel LM: Structure-based development of anticancer drugs: complexes of NAD(P)H:quinone oxidoreductase 1 with chemotherapeutic quinones.

    Structure 2001, 9(8):659-667. PubMed Abstract | Publisher Full Text OpenURL

  3. Bressi JC, Verlinde CL, Aronov AM, Shaw ML, Shin SS, Nguyen LN, Suresh S, Buckner FS, Van Voorhis WC, Kuntz ID, Hol WG, Gelb MH: Adenosine analogues as selective inhibitors of glyceraldehyde-3-phosphate dehydrogenase of Trypanosomatidae via structure-based drug design.

    J Med Chem 2001, 44(13):2080-2093. PubMed Abstract | Publisher Full Text OpenURL

  4. Newsletter 1984

    European Journal of Biochemistry 1984, 138(1):5-7. Publisher Full Text OpenURL

  5. Eckstein F: Nucleoside phosphorothioates.

    Annu Rev Biochem 1985, 54:367-402. PubMed Abstract | Publisher Full Text OpenURL

  6. Cech TR, Herschlag D, Piccirilli JA, Pyle AM: RNA catalysis by a group I ribozyme. Developing a model for transition state stabilization.

    J Biol Chem 1992, 267(25):17479-17482. PubMed Abstract | Publisher Full Text OpenURL

  7. Padgett RA, Podar M, Boulanger SC, Perlman PS: The stereochemical course of group II intron self-splicing.

    Science 1994, 266(5191):1685-1688. PubMed Abstract | Publisher Full Text OpenURL

  8. Domanico PL, Rahil JF, Benkovic SJ: Unambiguous stereochemical course of rabbit liver fructose bisphosphatase hydrolysis.

    Biochemistry 1985, 24(7):1623-1628. PubMed Abstract | Publisher Full Text OpenURL

  9. Tsai MD: Use of phosphorus-31 nuclear magnetic resonance to distinguish bridge and nonbridge oxygens of oxygen-17-enriched nucleoside triphosphates. Stereochemistry of acetate activation by acetyl coenzyme A synthetase.

    Biochemistry 1979, 18(8):1468-1472. PubMed Abstract | Publisher Full Text OpenURL

  10. Schultze P, Feigon J: Chirality errors in nucleic acid structures.

    Nature 1997, 387(6634):668. PubMed Abstract | Publisher Full Text OpenURL

  11. Waszkowycz B: Towards improving compound selection in structure-based virtual screening.

    Drug Discov Today 2008, 13(5–6):219-226. PubMed Abstract | Publisher Full Text OpenURL

  12. Good A: Structure-based virtual screening protocols.

    Curr Opin Drug Discov Devel 2001, 4(3):301-307. PubMed Abstract OpenURL

  13. Berthold CL, Toyota CG, Moussatche P, Wood MD, Leeper F, Richards NG, Lindqvist Y: Crystallographic snapshots of oxalyl-CoA decarboxylase give insights into catalysis by nonoxidative ThDP-dependent decarboxylases.

    Structure 2007, 15(7):853-861. PubMed Abstract | Publisher Full Text OpenURL

  14. Reuter K, Mofid MR, Marahiel MA, Ficner R: Crystal structure of the surfactin synthetase-activating enzyme sfp: a prototype of the 4'-phosphopantetheinyl transferase superfamily.

    The EMBO journal 1999, 18(23):6823-6831. PubMed Abstract | Publisher Full Text | PubMed Central Full Text OpenURL

  15. Thoden JB, Raushel FM, Wesenberg G, Holden HM: The binding of inosine monophosphate to Escherichia coli carbamoyl phosphate synthetase.

    J Biol Chem 1999, 274(32):22502-22507. PubMed Abstract | Publisher Full Text OpenURL

  16. Al-Karadaghi S, Cedergren-Zeppezauer ES, Hovmoller S: Refined crystal structure of liver alcohol dehydrogenase-NADH complex at 1.8 Å resolution.

    Acta Crystallogr D Biol Crystallogr 1994, 50:793-807. PubMed Abstract | Publisher Full Text OpenURL

  17. Carugo O, Argos P: NADP-dependent enzymes. I: Conserved stereochemistry of cofactor binding.

    Proteins 1997, 28(1):10-28. PubMed Abstract | Publisher Full Text OpenURL

  18. Henrick K, Feng Z, Bluhm WF, Dimitropoulos D, Doreleijers JF, Dutta S, Flippen-Anderson JL, Ionides J, Kamada C, Krissinel E, Lawson CL, Markley JL, Nakamura H, Newman R, Shimizu Y, Swaminathan J, Velankar S, Ory J, Ulrich EL, Vranken W, Westbrook J, Yamashita R, Yang H, Young J, Yousufuddin M, Berman HM: Remediation of the protein data bank archive.

    Nucleic Acids Res 2008, (36 Database):D426-433. PubMed Abstract | Publisher Full Text | PubMed Central Full Text OpenURL

  19. Allen FH: The Cambridge Structural Database: a quarter of a million crystal structures and rising.

    Acta Crystallogr B 2002, 58:380-388. PubMed Abstract | Publisher Full Text OpenURL

  20. Cahn RS, Ingold C, Prelog V: Specification of molecular chirality.

    Angew Chem Int Ed Engl 1966, 5(4):385-415. Publisher Full Text OpenURL

  21. Prelog V, Helmchen G: Basic principles of the CIP-system and proposals for a revision.

    Angew Chem Int Ed Engl 1982, 21(8):567-583. Publisher Full Text OpenURL

  22. Cieplak T, Wisniewski J: A new effective algorithm for the unambiguous identification of the stereochemical characteristics of compounds during their registration in databases.

    Molecules 2001, 6:915-926. OpenURL

  23. Emsley P, Cowtan K: Coot: model-building tools for molecular graphics.

    Acta Crystallogr D Biol Crystallogr 2004, 60(Pt 12 Pt 1):2126-2132. PubMed Abstract | Publisher Full Text OpenURL

  24. Krissinel E, Henrick K: Secondary-structure matching (SSM), a new tool for fast protein structure alignment in three dimensions.

    Acta Crystallogr D Biol Crystallogr 2004, 60(Pt 12 Pt 1):2256-2268. PubMed Abstract | Publisher Full Text OpenURL

  25. DeLano WL: The PyMOL molecular graphics system. [http://pymol.sourceforge.net] webcite

    San Carlos, CA, USA: DeLano Scientific; 2002.