Table 3

An example of an IDF.
Investigation Title	University of Heidelberg H sapiens TK6
Experimental Designs	genetic_modification_design	time_series_design
Experimental Factors	GeneticModification	Time

Person Last Name	Maier	Fleckenstein	Li
Person First Name	Patrick	Katharina	Li
Person Email	patrick.maier@radonk.ma.uni-heidelberg.de
Person Phone	+496213833773
Person Address	Theodor-Kutzer-Ufer 1–3
Person Affiliation	Department of Radiation Oncology, University of Heidelberg
Person Roles	submitter; investigator	investigator	investigator

Quality Control Types	biological_replicate
Replicate Types	biological_replicate
Date of Experiment	2005-02-28
Public Release Date	2006-01-03
PubMed ID	12345678
Publication Author List	Patrick Maier; Katharina Fleckenstein; Li Li; Stephanie Laufs; Jens Zeller; Stefan Fruehauf; Carsten Herskind; Frederik Wenz
Publication Status	submitted
Experiment Description	Gene expression of TK6 cells transduced with an oncoretrovirus expressing MDR1 (TK6MDR1) was compared to untransduced TK6 cells and to TK6 cell transduced with an oncoretrovirus expressing the Neomycin resistance gene (TK6neo). Two biological replicates of each were generated and the expression profiles were determined using Affymetrix Human Genome U133 Plus2.0 GeneChip microarrays. Comparisons between the sample groups allow the identification of genes with expression dependent on the MDR1 overexpression.

Protocol Name	GROWTHPRTCL10653	EXTPRTCL10654	TRANPRTCL10656
Protocol Type	grow	nucleic_acid_extraction	bioassay_data_transformation
Protocol Description	TK6 cells were grown in suspension cultures in RPMI 1640 medium supplemented with 10% horse serum (Invitrogen, Karlsruhe, Germany). The cells were routinely maintained at 37 C and 5% CO2.	Approximately 10 cells were lysed in RLT buffer (Qiagen).Total RNA was extracted from the cell lysate using an RNeasy kit (Qiagen).	Mixed Model Normalization with SAS Micro Array Solutions (version 1.3).
Protocol Parameters	media	Extracted Product; Amplification

SDRF Files	e-mexp-428_tab.txt

Database	CTO	MO	nci_meta
Database URI	http://obo.sourceforge.net/cgi-bin/detail.cgi?cell	http://mged.sourceforge.net/ontologies/MGEDontology.php	http://ncimeta.nci.nih.gov/indexMetaphrase.html
Database Version		1.3.0.1

The first column represents the qualifier name, while their values are given starting from column 2 (if the qualifier has two or more values, each is given in a separate column).
Rayner et al. BMC Bioinformatics 2006 7:489 doi:10.1186/1471-2105-7-489