Figure 3.

Example of intron detection in the human ATP1B1 (UG:Hs.291196) gene without (A) or with (B) the refinement of exon-intron boundaries. The first row shows the genomic sequence aligned to the EST sequences (below). In (A) four different introns are detected (A, B, C, D) that can be merged to only two (A, D) in B. Absolute coordinate (NCBI 35 assembly) are shown for each intron and acceptor/donor splice sites are in black-background.