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	1. 561 Accesses	Research article Side chain requirements for affinity and specificity in D5, an HIV-1 antibody derived from the V_H1-69 germline segment Alex Stewart, Joseph S Harrison, Lauren K Regula, Jonathan R Lai BMC Biochemistry 2013, 14:9 (8 April 2013) Abstract \| Full text \| PDF \| ePUB \| PubMed
	2. 487 Accesses	Research article The complete N-terminal extension of heparin cofactor II is required for maximal effectiveness as a thrombin exosite 1 ligand Amanda J Boyle, Leigh Ann Roddick, Varsha Bhakta, Melissa D Lambourne, Murray S Junop, Patricia C Liaw, Jeffrey I Weitz, William P Sheffield BMC Biochemistry 2013, 14:6 (7 March 2013) Abstract \| Full text \| PDF \| PubMed \| Editor’s summary An isolated form of N-terminal acidic extension of Heparin cofactor II (HCII 1-75) binds thrombin with a higher affinity than glycosaminoglycans (GAGs), suggesting that this region of Heparin cofactor II (HCII) is required for binding of thrombin exosite 1.
	3. 455 Accesses	Research article Construction of a chimeric thermoacidophilic beta-endoglucanase Kristina Kufner, Georg Lipps BMC Biochemistry 2013, 14:11 (29 April 2013) Abstract \| Full text \| PDF \| ePUB \| PubMed
	4. 402 Accesses	Reviewer acknowledgement Annual acknowledgement of manuscript reviewers Emilie Aime BMC Biochemistry 2013, 14:7 (21 March 2013) Abstract \| Full text \| PDF \| ePUB \| PubMed
	5. 388 Accesses	Research article Biochemical characterization of three putative ATPases from a new type IV secretion system of Aeromonas veronii plasmid pAC3249A Ashraf Y Rangrez, Mohammad Y Abajy, Walter Keller, Yogesh Shouche, Elisabeth Grohmann BMC Biochemistry 2010, 11:10 (9 February 2010) Abstract \| Full text \| PDF \| PubMed
	6. 370 Accesses	Research article Glutathionylation of beta-actin via a cysteinyl sulfenic acid intermediary Magnus Johansson, Mathias Lundberg BMC Biochemistry 2007, 8:26 (10 December 2007) Abstract \| Full text \| PDF \| PubMed
	7. 327 Accesses	Research article Nedd8 processing enzymes in Schizosaccharomyces pombe Jean E O’Donoghue, Dawadschargal Bech-Otschir, Ida B Larsen, Mairi Wallace, Rasmus Hartmann-Petersen, Colin Gordon BMC Biochemistry 2013, 14:8 (15 March 2013) Abstract \| Full text \| PDF \| PubMed
	8. 300 Accesses	Methodology article A simplified method for analysis of polyunsaturated fatty acids Jing X Kang, Jingdong Wang BMC Biochemistry 2005, 6:5 (24 March 2005) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central
	9. 267 Accesses	Research article Metazoan-like signaling in a unicellular receptor tyrosine kinase Kira P Schultheiss, Barbara P Craddock, Michael Tong, Markus Seeliger, W Todd Miller BMC Biochemistry 2013, 14:4 (12 February 2013) Abstract \| Full text \| PDF \| PubMed \| Editor’s summary RTKB2 kinase from the unicellular choanoflagellate Monosiga brevicollis demonstrates regulatory functions as complex as those performed by mammalian receptor tyrosine kinases (RTKs), showing that complex signal transduction circuitry predates the evolution of metazoans.
	10. 261 Accesses	Review Role of the ubiquitin proteasome system in Parkinson's disease Kah-Leong Lim, Jeanne MM Tan BMC Biochemistry 2007, 8(Suppl 1):S13 (22 November 2007) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central
	11. 251 Accesses	Research article Mapping of the minimal inorganic phosphate transporting unit of human PiT2 suggests a structure universal to PiT-related proteins from all kingdoms of life Pernille Bøttger, Lene Pedersen BMC Biochemistry 2011, 12:21 (17 May 2011) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central \| 1 comment
	12. 243 Accesses	Research article Expression, purification and biochemical characterization of Schizosaccharomyces pombe Mcm4, 6 and 7 Meng Xu, Y Paul Chang, Xiaojiang S Chen BMC Biochemistry 2013, 14:5 (27 February 2013) Abstract \| Full text \| PDF \| ePUB \| PubMed
	13. 240 Accesses	Research article Characterization of a β-glucanase produced by Rhizopus microsporus var. microsporus, and its potential for application in the brewing industry Klecius Celestino, Ricardo B Cunha, Carlos R Felix BMC Biochemistry 2006, 7:23 (5 December 2006) Abstract \| Full text \| PDF \| PubMed
	14. 237 Accesses	Research article Characterization of methionine oxidation and methionine sulfoxide reduction using methionine-rich cysteine-free proteins Xinwen Liang, Alaattin Kaya, Yan Zhang, Dung Le, Deame Hua, Vadim N Gladyshev BMC Biochemistry 2012, 13:21 (23 October 2012) Abstract \| Full text \| PDF \| PubMed
	15. 236 Accesses	Research article Fatty acyl-CoA reductases of birds Janine Hellenbrand, Eva-Maria Biester, Jens Gruber, Mats Hamberg, Margrit Frentzen BMC Biochemistry 2011, 12:64 (12 December 2011) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central \| Editor’s summary Fatty acyl-CoA reductases (FARs) cloned from the preening glands of barn owls, chickens and geese represent two distinct groups of isozymes that may be involved in wax ester biosynthesis and other pathways such as ether lipid synthesis
	16. 234 Accesses	Research article Presenilin-1 regulates the constitutive turnover of the fibronectin matrix in endothelial cells Rita De Gasperi, Miguel A Gama Sosa, Gregory A Elder BMC Biochemistry 2012, 13:28 (21 December 2012) Abstract \| Full text \| PDF \| PubMed
	17. 228 Accesses	Research article Monitoring compartment-specific substrate cleavage by cathepsins B, K, L, and S at physiological pH and redox conditions Silvia Jordans, Saša Jenko-Kokalj, Nicole M Kühl, Sofia Tedelind, Wolfgang Sendt, Dieter Brömme, Dušan Turk, Klaudia Brix BMC Biochemistry 2009, 10:23 (22 September 2009) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central \| Editor’s summary Cathepsin cysteine proteases are active under conditions simulating extracellular and intracellular compartments with a range of pHs and redox potentials, as shown by cleavage of the natural substrate thyroglobulin in distinct ways in different physiologic environments.
	18. 215 Accesses	Research article Ski-interacting protein (SKIP) interacts with androgen receptor in the nucleus and modulates androgen-dependent transcription Daniel Abankwa, Susan M Millard, Nick Martel, Catherine S Choong, Miao Yang, Lisa M Butler, Grant Buchanan, Wayne D Tilley, Nobuhide Ueki, Michael J Hayman, Gary M Leong BMC Biochemistry 2013, 14:10 (8 April 2013) Abstract \| Provisional PDF \| PubMed
	19. 212 Accesses	Research article Acetylcholinesterase assay for cerebrospinal fluid using bupivacaine to inhibit butyrylcholinesterase Wolfram H Kluge, Harald H Kluge, Heike I Bauer, Stefan Pietsch, Jens Anders, Rudolf A Venbrocks BMC Biochemistry 2001, 2:17 (21 December 2001) Abstract \| Full text \| PDF \| PubMed
	20. 212 Accesses	Research article Regulation of the BRCA1 gene by an SRC3/53BP1 complex Dale Corkery, Gobi Thillainadesan, Niamh Coughlan, Ryan D Mohan, Majdina Isovic, Marc Tini, Joseph Torchia BMC Biochemistry 2011, 12:50 (13 September 2011) Abstract \| Full text \| PDF \| PubMed \| Editor’s summary The tumor suppressor 53BP1 and the oncogene SRC3 occupy the same region of the BRCA1 promoter and are both required for BRCA1 expression, suggesting that this complex plays an important role in DNA repair and genomic stability
	21. 210 Accesses	Research article Enzymatic properties of Staphylococcus aureus adenosine synthase (AdsA) Vilasack Thammavongsa, Olaf Schneewind, Dominique M Missiakas BMC Biochemistry 2011, 12:56 (28 October 2011) Abstract \| Full text \| PDF \| PubMed
	22. 206 Accesses	Research article Phylogenetic and experimental characterization of an acyl-ACP thioesterase family reveals significant diversity in enzymatic specificity and activity Fuyuan Jing, David C Cantu, Jarmila Tvaruzkova, Jay P Chipman, Basil J Nikolau, Marna D Yandeau-Nelson, Peter J Reilly BMC Biochemistry 2011, 12:44 (10 August 2011) Abstract \| Full text \| PDF \| PubMed
	23. 204 Accesses	Research article Application of Celluspots peptide arrays for the analysis of the binding specificity of epigenetic reading domains to modified histone tails Ina Bock, Srikanth Kudithipudi, Raluca Tamas, Goran Kungulovski, Arunkumar Dhayalan, Albert Jeltsch BMC Biochemistry 2011, 12:48 (31 August 2011) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central
	24. 200 Accesses	Research article Arg188 in rice sucrose transporter OsSUT1 is crucial for substrate transport Ye Sun, John M Ward BMC Biochemistry 2012, 13:26 (21 November 2012) Abstract \| Full text \| PDF \| PubMed \| Editor’s summary Arginine-188 of the plant sucrose uptake transporter OsSUT1 is required for substrate translocation but not interaction, implicating the involvement of an alternative substrate binding site in substrate recognition.
	25. 199 Accesses	Research article Purification and characterization of cysteine protease from germinating cotyledons of horse gram Rajeswari Jinka, Vadde Ramakrishna, Sridhar K Rao, Ramakrishna P Rao BMC Biochemistry 2009, 10:28 (17 November 2009) Abstract \| Full text \| PDF \| PubMed
	26. 199 Accesses	Database The Thiamine diphosphate dependent Enzyme Engineering Database: A tool for the systematic analysis of sequence and structure relations Michael Widmann, Robert Radloff, Jürgen Pleiss BMC Biochemistry 2010, 11:9 (1 February 2010) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central \| Editor’s summary A database of thiamine diphosphate (ThDP) dependent enzymes includes multisequence alignments and phylogenetic trees for families of the enzymes, structural information, and SNPs of human enzymes that might be related to disease.
	27. 192 Accesses	Research article Characterization of the aggregates formed during recombinant protein expression in bacteria Andrea Schrödel, Ario de Marco BMC Biochemistry 2005, 6:10 (31 May 2005) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central
	28. 193 Accesses	Research article The roles of aldehyde dehydrogenases (ALDHs) in the PDH bypass of Arabidopsis Yanling Wei, Ming Lin, David J Oliver, Patrick S Schnable BMC Biochemistry 2009, 10:7 (25 March 2009) Abstract \| Full text \| PDF \| PubMed
	29. 191 Accesses	Research article The COP1 E3-ligase interacts with FIP200, a key regulator of mammalian autophagy Saori Kobayashi, Noriko Yoneda-Kato, Nagisa Itahara, Akihiro Yoshida, Jun-ya Kato BMC Biochemistry 2013, 14:1 (6 January 2013) Abstract \| Full text \| PDF \| PubMed
	30. 189 Accesses	Research article Effects of low frequency ultrasound on some properties of fibrinogen and its plasminolysis Eugene A Cherniavsky, Igor S Strakha, Igor E Adzerikho, Vladimir M Shkumatov BMC Biochemistry 2011, 12:60 (23 November 2011) Abstract \| Full text \| PDF \| PubMed
	31. 188 Accesses	Research article Characterization of rubber particles and rubber chain elongation in Taraxacum koksaghyz Thomas Schmidt, Malte Lenders, Andrea Hillebrand, Nicole van Deenen, Oliver Munt, Rudolf Reichelt, Wolfgang Eisenreich, Rainer Fischer, Dirk Prüfer, Christian Gronover BMC Biochemistry 2010, 11:11 (19 February 2010) Abstract \| Full text \| PDF \| PubMed
	32. 186 Accesses	Research article Extraction and inhibition of enzymatic activity of botulinum neurotoxins /B1, /B2, /B3, /B4, and /B5 by a panel of monoclonal anti-BoNT/B antibodies Suzanne R Kalb, Wanda I Santana, Isin N Geren, Consuelo Garcia-Rodriguez, Jianlong Lou, Theresa J Smith, James D Marks, Leonard A Smith, James L Pirkle, John R Barr BMC Biochemistry 2011, 12:58 (15 November 2011) Abstract \| Full text \| PDF \| PubMed
	33. 185 Accesses	Research article Two chitinase-like proteins abundantly accumulated in latex of mulberry show insecticidal activity Sakihito Kitajima, Kaeko Kamei, Shigeru Taketani, Masamitsu Yamaguchi, Fumiko Kawai, Aino Komatsu, Yoshihiro Inukai BMC Biochemistry 2010, 11:6 (28 January 2010) Abstract \| Full text \| PDF \| PubMed \| 1 comment
	34. 184 Accesses	Research article In vitro substrate phosphorylation by Ca²⁺/calmodulin-dependent protein kinase kinase using guanosine-5^′-triphosphate as a phosphate donor Saki Yurimoto, Tomohito Fujimoto, Masaki Magari, Naoki Kanayama, Ryoji Kobayashi, Hiroshi Tokumitsu BMC Biochemistry 2012, 13:27 (5 December 2012) Abstract \| Full text \| PDF \| PubMed
	35. 181 Accesses	Research article The effects of NaCl concentration and pH on the stability of hyperthermophilic protein Ssh10b Yong-Jin Mao, Xiang-Rong Sheng, Xian-Ming Pan BMC Biochemistry 2007, 8:28 (21 December 2007) Abstract \| Full text \| PDF \| PubMed
	36. 175 Accesses	Research article Analysis of phosphorylation of human heat shock factor 1 in cells experiencing a stress Toumy Guettouche, Frank Boellmann, William S Lane, Richard Voellmy BMC Biochemistry 2005, 6:4 (11 March 2005) Abstract \| Full text \| PDF \| PubMed \| 1 comment \| Editor’s summary Alanine scanning and mass spectrometry analysis identify phosphorylation of twelve serine residues in human heat shock factor 1 upon heat stress and further functional studies suggest serine-326 plays a critical part in activation of the factor's transcriptional competence
	37. 169 Accesses	Review The ubiquitin system, disease, and drug discovery Matthew D Petroski BMC Biochemistry 2008, 9(Suppl 1):S7 (21 October 2008) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central
	38. 173 Accesses	Research article The major leucyl aminopeptidase of Trypanosoma cruzi (LAPTc) assembles into a homohexamer and belongs to the M17 family of metallopeptidases Gloria Cadavid-Restrepo, Thiago S Gastardelo, Eric Faudry, Hugo de Almeida, Izabela MD Bastos, Raquel S Negreiros, Meire M Lima, Teresa C Assumpção, Keyla C Almeida, Michel Ragno, Christine Ebel, Bergmann M Ribeiro, Carlos R Felix, Jaime M Santana BMC Biochemistry 2011, 12:46 (23 August 2011) Abstract \| Full text \| PDF \| PubMed \| Editor’s summary The major leucyl aminopeptidase of the protozoan parasite Trypanosoma cruzi is a homohexameric metalloproteinase belonging to the M17 peptidase family, and could have a function in the nutritional supply of essential amino acids
	39. 167 Accesses	Review Role of proteasomes in disease Burkhardt Dahlmann BMC Biochemistry 2007, 8(Suppl 1):S3 (22 November 2007) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central
	40. 164 Accesses	Research article Cluster analysis application identifies muscle characteristics of importance for beef tenderness Sghaier Chriki, Graham E Gardner, Catherine Jurie, Brigitte Picard, Didier Micol, Jean-Paul Brun, Laurent Journaux, Jean-Francois Hocquette BMC Biochemistry 2012, 13:29 (22 December 2012) Abstract \| Full text \| PDF \| PubMed
	41. 160 Accesses	Research article A new classification system for bacterial Rieske non-heme iron aromatic ring-hydroxylating oxygenases Ohgew Kweon, Seong-Jae Kim, Songjoon Baek, Jong-Chan Chae, Michael D Adjei, Dong-Heon Baek, Young-Chang Kim, Carl E Cerniglia BMC Biochemistry 2008, 9:11 (3 April 2008) Abstract \| Full text \| PDF \| PubMed
	42. 159 Accesses	Methodology article Streptavidin-Binding Peptide (SBP)-tagged SMC2 allows single-step affinity fluorescence, blotting or purification of the condensin complex Ji Hun Kim, Tsz M Chang, Alison N Graham, K H Andy Choo, Paul Kalitsis, Damien F Hudson BMC Biochemistry 2010, 11:50 (31 December 2010) Abstract \| Full text \| PDF \| PubMed
	43. 154 Accesses	Research article The FTO (fat mass and obesity associated) gene codes for a novel member of the non-heme dioxygenase superfamily Luis Sanchez-Pulido, Miguel A Andrade-Navarro BMC Biochemistry 2007, 8:23 (8 November 2007) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central
	44. 155 Accesses	Research article Protein phosphatase 2a (PP2A) binds within the oligomerization domain of striatin and regulates the phosphorylation and activation of the mammalian Ste20-Like kinase Mst3 Johnthan Gordon, Juyeon Hwang, Karma J Carrier, Candace A Jones, Quiana L Kern, Carlos S Moreno, Richard H Karas, David C Pallas BMC Biochemistry 2011, 12:54 (10 October 2011) Abstract \| Full text \| PDF \| PubMed
	45. 154 Accesses	Research article The DNA polymerase activity of Pol ε holoenzyme is required for rapid and efficient chromosomal DNA replication in Xenopus egg extracts Koh Shikata, Taro Sasa-Masuda, Yukiko Okuno, Shou Waga, Akio Sugino BMC Biochemistry 2006, 7:21 (22 August 2006) Abstract \| Full text \| PDF \| PubMed
	46. 151 Accesses	Research article The molecular basis of electroporation D Peter Tieleman BMC Biochemistry 2004, 5:10 (19 July 2004) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central \| F1000 Biology \| Editor’s summary A molecular model of electroporation, based on molecular dynamics simulations of phosphoplipid bilayers, demonstrates that strong electric field gradients provide a driving force for water penetration into membranes.
	47. 151 Accesses	Hypothesis Sensor potency of the moonlighting enzyme-decorated cytoskeleton: the cytoskeleton as a metabolic sensor Vic Norris, Patrick Amar, Guillaume Legent, Camille Ripoll, Michel Thellier, Judit Ovádi BMC Biochemistry 2013, 14:3 (11 February 2013) Abstract \| Full text \| PDF \| PubMed
	48. 147 Accesses	Research article Measurement of peroxisomal enzyme activities in the liver of brown trout (Salmo trutta), using spectrophotometric methods Maria Rocha, Eduardo Rocha, Albina D Resende, Alexandre Lobo-da-Cunha BMC Biochemistry 2003, 4:2 (11 March 2003) Abstract \| Full text \| PDF \| PubMed
	49. 146 Accesses	Methodology article Optimization of a direct spectrophotometric method to investigate the kinetics and inhibition of sialidases Jasvinder Hayre, Guogang Xu, Luisa Borgianni, Garry L Taylor, Peter W Andrew, Jean-Denis Docquier, Marco R Oggioni BMC Biochemistry 2012, 13:19 (2 October 2012) Abstract \| Full text \| PDF \| PubMed
	50. 141 Accesses	Research article Metabolic signature of breast cancer cell line MCF-7: profiling of modified nucleosides via LC-IT MS coupling Dino Bullinger, Hans Neubauer, Tanja Fehm, Stefan Laufer, Christoph H Gleiter, Bernd Kammerer BMC Biochemistry 2007, 8:25 (29 November 2007) Abstract \| Full text \| PDF \| PubMed \| Cited on BioMed Central