Major pathways involved in nebivolol-stimulated NO and ONOO- generation, and maximal endothelial NO concentration stimulated with nebivolol alone or following an incubation with several modulators. (A) Maximal NO concentration release from HUVECs stimulated with nebivolol (1 μM). Cells were also incubated with suramin (10 μM), Gd3+ (200 μM), SR59230A (1 μM), L-748,337 (3 μM) and suramin + SR59230A for 30 minutes and NO release was stimulated with nebivolol (1 μM). Values are mean ± SD (n = 5). One-way ANOVA, Student-Newman-Keuls multiple comparison post-hoc test: *P < 0.05 compared with nebivolol alone. (B) Nebivolol-stimulated generation of NO and ONOO- involves at least two major pathways and several steps. (C) At low nebivolol concentration, K4 will be more determinant than at higher concentration. Values are mean ± SD (n = 5). Student’s t-test: *P < 0.05 compared with K1 + K2 + K3.
Mason et al. BMC Pharmacology and Toxicology 2013 14:48 doi:10.1186/2050-6511-14-48