Histology, Immunohistochemistry of Pancreas and metabolic parameters in male ZDF rats. A-C: H and E stained pancreatic Islets sections from Lean control, ZDF control and CNX-011-67 treatment group. Magnifications at 400X. Islets with normal architecture from Lean control (A,B) and with distorted architecture in ZDF animals D-F: Immunofluorescent staining for insulin in consecutive sections of Pancreas. Immunostaining intensity of B-cells for Insulin is good in Lean control, poor in ZDF control and improved in Treatment group. G-I: Double immuno fluorescence staining for PDX1 (red) and insulin (green) of rat pancreatic islets. Compared to ZDF control the number of PDX1 positive nucleus (red) in the treatment appears to be increased. J-L: Brown colored apoptotic beta cells (arrow) in a pancreatic islet. Compared to ZDF control, apoptotic beta cells in treatment are found to be reduced.
Gowda et al. BMC Pharmacology and Toxicology 2013 14:28 doi:10.1186/2050-6511-14-28