Drug-induced changes in DNA methylation levels of repetitive elements. DNA methylation levels of the three classes of repeat sequences Alu, Satα, and Sat2, assessed by specific MethyLight assays, significantly decreased in Huh-7 cells upon treatment with AZA. The degree of hypomethylation correlated with drug concentration, with the exception of an increase in DNA methylation seen for all three repetitive elements at the 20 μM AZA concentration.
Gertych et al. BMC Pharmacology and Toxicology 2013 14:11 doi:10.1186/2050-6511-14-11