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Single-molecule photobleaching reveals increased MET receptor dimerization upon ligand binding in intact cells

Marina S Dietz1, Daniel Haße2, Davide M Ferraris3, Antonia Göhler4, Hartmut H Niemann2* and Mike Heilemann1*

Author Affiliations

1 Institute of Physical and Theoretical Chemistry, Johann Wolfgang Goethe-University, Max-von-Laue-Str. 7, 60438 Frankfurt, Germany

2 Structural Biochemistry, Department of Chemistry, Bielefeld University, Universitaetsstr. 25, 33615 Bielefeld, Germany

3 Department of Pharmaceutical Science, University of Eastern Piedmont “Amedeo Avogadro”, Via Bovio 6, Novara, 28100, Italy

4 Department of Biotechnology and Biophysics, Julius-Maximilians University, Am Hubland, Biozentrum, Wuerzburg, 97074, Germany

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BMC Biophysics 2013, 6:6  doi:10.1186/2046-1682-6-6

Published: 3 June 2013

Additional files

Additional file 1: Figure S1:

dSTORM imaging of MET receptor in fixed HeLa cells. MET was immunostained with Alexa Fluor 647. (A) Comparison of wide field and super-resolution dSTORM images (scale bar 2 μm). (B) Enlarged section (2 × 2 μm²) of the inset in (A), (C) dSTORM image of the section in (B).

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