Email updates

Keep up to date with the latest news and content from BMC Biophysics and BioMed Central.

Open Access Research article

Differences in adhesion and protrusion properties correlate with differences in migration speed under EGF stimulation

Yue Hou1, Sarah Hedberg3 and Ian C Schneider12*

Author Affiliations

1 Department of Chemical and Biological Engineering, Iowa State University, Iowa, USA

2 Department of Genetics, Development and Cell Biology, Iowa State University, Iowa, USA

3 Department of Biochemical Engineering, University College London, London, UK

For all author emails, please log on.

BMC Biophysics 2012, 5:8  doi:10.1186/2046-1682-5-8

Published: 11 May 2012

Additional files

Additional file 1:

Figure S1.Migration trajectories of typical cells under different EGF concentrations. Three cell tracks were chosen randomly under each EGF concentrations and labeled with different colors. All trajectories were aligned to the starting point (0, 0).

Format: EPS Size: 1009KB Download file

Open Data

Additional file 2:

Figure S2.Paxillin-EGFP expression levels for cells with different cell speeds and under different EGF stimulation conditions. Mean intensity of individual cells as a function of A. cell speed, and B. EGF concentrations. Ncell = 56.

Format: EPS Size: 517KB Download file

Open Data

Additional file 3:

Figure S3.Average paxillin-EGFP intensity in cells as a function of time. A. Mean FA intensity and B. mean intensity of the whole cell for all cells as a function of time. Mean FA Intensity: Ncells = 55. Mean Intensity Ncells = 53.

Format: EPS Size: 632KB Download file

Open Data

Additional file 4:

Time-resolved data of mean FA intensity and FA number per cell. A. Mean FA intensity and C. FA number per cell for the cell shown in Figure 2 under 0.01 nM EGF stimulation as a function of time. B. FA mean intensity and D. number per cell of five typical cells under different EGF concentrations as a function of time.

Format: EPS Size: 768KB Download file

Open Data

Additional file 5:

Figure S5.Distributions of FA size, speed, lifetime and elongation for different EGF stimulation conditions. Histograms of FA size A.-C., speed E.-G., lifetime I.-K. and size M.-O. were generated by dividing cells into three EGF stimulation groups (A., E., I. and M., no EGF (0 nM), B., F., J. and N. low EGF (0.01 and 0.1 nM) and C., G.,K. and O. high EGF (1, 10 and 100 nM)). Histograms were fitted with lognormal probability distributions except for speed, which was fitted with a Weibull probability distribution. The mean values of FA D. size, H. speed, L. lifetime and P. elongation are also shown. The number of measurements of FA properties is the product of the average FA number and the cell number. Size, speed, lifetime and elongation: Ncell,no = 5, NFA,no = 1963, Ncell,low = 20, NFA,low = 13,024, Ncell,high = 30, NFA,high = 14,648. Error bars are 95 % confidence intervals and asterisks denote p < 0.01.

Format: EPS Size: 2.2MB Download file

Open Data

Additional file 6:

Figure S6.Distributions of FA size, speed, lifetime and elongation for slow and fast migrating cells. Histograms of FA size A.-B., speed D.-E., lifetime G.-H. and size J.-K. were generated by dividing cells into slow and fast migrating groups (A., D., G. and J. slow migrating cells and B., E., H. and K. fast migrating cells). Histograms were fitted with lognormal probability distributions except for speed, which was fitted with a Weibull probability distribution. The mean values of FA C. size, F. speed, I. lifetime and L. elongation are also shown. The number of measurements of FA properties is the product of the average FA number and the cell number. Size, speed, lifetime and elongation: Ncell,slow = 21, NFA,slow = 12,773, Ncell,fast = 34, NFA,fast = 16,842. Error bars are 95 % confidence intervals and asterisks denote p < 0.01.

Format: EPS Size: 1.7MB Download file

Open Data