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Open Access Research article

Comparison of microbial populations in the small intestine, large intestine and feces of healthy horses using terminal restriction fragment length polymorphism

Angelika Schoster12*, Luis Guillermo Arroyo1, Henry Rolf Staempfli1 and Jeffrey Scott Weese1

Author Affiliations

1 Department of Clinical Studies, Ontario Veterinary College, University of Guelph, Guelph, Canada

2 Equine Department, Vetsuisse Faculty, Clinic for Equine Internal Medicine, University of Zurich, Winterthurerstrsse 260, Zurich, 8750, Switzerland

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BMC Research Notes 2013, 6:91  doi:10.1186/1756-0500-6-91

Published: 12 March 2013

Abstract

Background

The composition of the microbiota of the equine intestinal tract is complex. Determining whether the microbial composition of fecal samples is representative of proximal compartments of the digestive tract could greatly simplify future studies. The objectives of this study were to compare the microbial populations of the duodenum, ileum, cecum, colon and rectum (feces) within and between healthy horses, and to determine whether rectal (fecal) samples are representative of proximal segments of the gastrointestinal tract. Intestinal samples were collected from ten euthanized horses. 16S rRNA gene PCR-based TRFLP was used to investigate microbiota richness in various segments of the gastrointestinal tract, and dice similarity indices were calculated to compare the samples.

Results

Within horses large variations of microbial populations along the gastrointestinal tract were seen. The microbiota in rectal samples was only partially representative of other intestinal compartments. The highest similarity was obtained when feces were compared to the cecum. Large compartmental variations were also seen when microbial populations were compared between six horses with similar dietary and housing management.

Conclusion

Rectal samples were not entirely representative of intestinal compartments in the small or large intestine. This should be taken into account when designing studies using fecal sampling to assess other intestinal compartments. Similarity between horses with similar dietary and husbandry management was also limited, suggesting that parts of the intestinal microbiota were unique to each animal in this study.

Keywords:
Equine intestinal microbiota; TRFLP; Intestinal compartments; Fecal bacteria