Effect of normalization options on PTGS2 gene expression in cervical tissues. Example of target gene expression levels in (A) cervical epithelial tissues and (B) cervical stromal tissues of the target gene when normalized to either the least stable (white), the top two most stable (gray) or the top three most stable (black) reference genes for each tissue type as determined with geNorm. Tissue samples from five guinea pigs representing various hormonal statuses; not pregnant (NP), mid pregnancy (MidP), late pregnancy not ripe (LP-NR), late pregnancy ripe (LP-R), and postpartum (PP). Error bars indicate 95% confidence interval; *, p<0.05; **, p<0.01; n=6.
Lindqvist et al. BMC Research Notes 2013 6:34 doi:10.1186/1756-0500-6-34