Pairwise variation of reference gene candidates in cervical cells and tissues as determined by geNorm. The first bar (2/3) represents the pairwise variation (V) in a 2 vs. 3 reference genes comparison. The second bar (3/4) represents pairwise variations in a 3 vs. 4 reference gene comparison. Variation values were determined using from 2/3 up to 13/14 reference gene comparisons in cultured stromal cells (A), cultured epithelial cells (B), stromal tissues (C), and epithelial tissues (D). Pairwise variation values of ≤0.15 were considered sufficient for normalization, values above 0.15 are considered too variable. Note increased V values in tissues compared with cells in culture.
Lindqvist et al. BMC Research Notes 2013 6:34 doi:10.1186/1756-0500-6-34