Primer validation. Each qPCR reaction mix was loaded in duplicate on 2% Agarose-1000 gels. First RPLP0 primer pair tested (gel B) resulted in an amplification product of ~130 bp (should be 61 bp). New primers were designed, and the resulting qPCR reaction was found to be of the correct size, 64 bp (gel C and D).
Lindqvist et al. BMC Research Notes 2013 6:34 doi:10.1186/1756-0500-6-34