A virus-induced gene silencing method to study soybean cyst nematode parasitism in Glycine max
1 Division of Plant Sciences, Christopher S. Bond Life Sciences Center and Interdisciplinary Plant Group, University of Missouri, Columbia, MO 65211, USA
2 Department of Plant Pathology and Microbiology, Iowa State University, Ames, IA 50011, USA
3 Current address: Pioneer Hi-Bred, Johnston IA, 50131, USA
4 Current address: Beadle Center for Biotechnology, University of Nebraska, Lincoln NE, 68588, USA
BMC Research Notes 2013, 6:255 doi:10.1186/1756-0500-6-255Published: 6 July 2013
Bean pod mottle virus (BPMV) based virus-induced gene silencing (VIGS) vectors have been developed and used in soybean for the functional analysis of genes involved in disease resistance to foliar pathogens. However, BPMV-VIGS protocols for studying genes involved in disease resistance or symbiotic associations with root microbes have not been developed.
Here we describe a BPMV-VIGS protocol suitable for reverse genetic studies in soybean roots. We use this method for analyzing soybean genes involved in resistance to soybean cyst nematode (SCN). A detailed SCN screening pipeline is described.
The VIGS method described here provides a new tool to identify genes involved in soybean-nematode interactions. This method could be adapted to study genes associated with any root pathogenic or symbiotic associations.