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Open Access Technical Note

A virus-induced gene silencing method to study soybean cyst nematode parasitism in Glycine max

Pramod K Kandoth1, Robert Heinz1, Greg Yeckel13, Nathan W Gross14, Parijat S Juvale2, John Hill2, Steven A Whitham2, Thomas J Baum2 and Melissa G Mitchum1*

Author Affiliations

1 Division of Plant Sciences, Christopher S. Bond Life Sciences Center and Interdisciplinary Plant Group, University of Missouri, Columbia, MO 65211, USA

2 Department of Plant Pathology and Microbiology, Iowa State University, Ames, IA 50011, USA

3 Current address: Pioneer Hi-Bred, Johnston IA, 50131, USA

4 Current address: Beadle Center for Biotechnology, University of Nebraska, Lincoln NE, 68588, USA

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BMC Research Notes 2013, 6:255  doi:10.1186/1756-0500-6-255

Published: 6 July 2013

Abstract

Background

Bean pod mottle virus (BPMV) based virus-induced gene silencing (VIGS) vectors have been developed and used in soybean for the functional analysis of genes involved in disease resistance to foliar pathogens. However, BPMV-VIGS protocols for studying genes involved in disease resistance or symbiotic associations with root microbes have not been developed.

Findings

Here we describe a BPMV-VIGS protocol suitable for reverse genetic studies in soybean roots. We use this method for analyzing soybean genes involved in resistance to soybean cyst nematode (SCN). A detailed SCN screening pipeline is described.

Conclusions

The VIGS method described here provides a new tool to identify genes involved in soybean-nematode interactions. This method could be adapted to study genes associated with any root pathogenic or symbiotic associations.

Keywords:
Heterodera glycines; Resistance; SCN; Soybean; Cyst nematode; Resistance; Virus-induced gene silencing; VIGS