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A comparative study on the antioxidant activity of methanolic extracts from different parts of Morus alba L. (Moraceae)

Muhammad Ali Khan1, Aziz Abdur Rahman1, Shafiqul Islam1, Proma Khandokhar2, Shahnaj Parvin1, Md Badrul Islam3, Mosharrof Hossain4, Mamunur Rashid1, Golam Sadik1, Shamima Nasrin1, M Nurul Haque Mollah5 and AHM Khurshid Alam1*

Author Affiliations

1 Department of Pharmacy, University of Rajshahi, Rajshahi, 6205, Bangladesh

2 Department of Pharmaceutical and Biological Sciences, UCL School of Pharmacy, London, UK

3 Senior Scientific Officer, BCSIR, Rajshahi, 6205, Bangladesh

4 Department of Zoology, University of Rajshahi, Rajshahi, 6205, Bangladesh

5 Department of Statistics, University of Rajshahi, Rajshahi, 6205, Bangladesh

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BMC Research Notes 2013, 6:24  doi:10.1186/1756-0500-6-24

Published: 19 January 2013



Antioxidants play an important role to protect damage caused by oxidative stress (OS). Plants having phenolic contents are reported to possess antioxidant properties. The present study was designed to investigate the antioxidant properties and phenolic contents (total phenols, flavonoids, flavonols and proanthrocyanidins) of methanolic extracts from Morus alba (locally named as Tut and commonly known as white mulberry) stem barks (TSB), root bark (TRB), leaves (TL) and fruits (TF) to make a statistical correlation between phenolic contents and antioxidant potential.


The antioxidant activities and phenolic contents of methanolic extractives were evaluated by in vitro standard method using spectrophotometer. The antioxidant activities were determined by total antioxidant capacity, DPPH (1,1-diphenyl-2-picrylhydrazine) radical scavenging assay, hydroxyl radical scavenging assay, ferrous reducing antioxidant capacity and lipid peroxidation inhibition assay methods.


Among the extracts, TSB showed the highest antioxidant activity followed by TRB, TF and TL. Based on DPPH and hydroxyl radical scavenging activity, the TSB extract was the most effective one with IC50 37.75 and 58.90 μg/mL, followed by TRB, TF and TL with IC50 40.20 and 102.03; 175.01 and 114.63 and 220.23 and 234.63 μg/mL, respectively. The TSB extract had the most potent inhibitory activity against lipid peroxidation with IC50 145.31 μg/mL. In addition, the reducing capacity on ferrous ion was in the following order: TSB > TRB > TL > TF. The content of phenolics, flavonoids, flavonols and proanthocyanidins of TSB was found to be higher than other extractives.


The results indicate high correlation and regression (p-value <0 .001) between phenolic contents and antioxidant potentials of the extracts, hence the Tut plant could serve as effective free radical inhibitor or scavenger which may be a good candidate for pharmaceutical plant-based products. However, further exploration is necessary for effective use in both modern and traditional system of medicines.

Morus alba; Moraceae; Oxidative stress; Antioxidant; Correlation and regression