Open Access Highly Accessed Research article

Estimation of total phenol and in vitro antioxidant activity of Albizia procera leaves

Mahfuza khatoon1, Ekramul Islam1, Rafikul Islam2, Aziz Abdur Rahman1, AHM Khurshid Alam1, Proma Khondkar3, Mamunur Rashid1 and Shahnaj Parvin1*

Author Affiliations

1 Department of Pharmacy, University of Rajshahi, Rajshahi-6205, Bangladesh

2 Department of Pharmacy, International Islamic University of Chittagong, Chittagong, 4203, Bangladesh

3 Department of Pharmaceutical and Biological Sciences, UCL School of Pharmacy, London, UK

For all author emails, please log on.

BMC Research Notes 2013, 6:121  doi:10.1186/1756-0500-6-121

Published: 27 March 2013



Research on natural products has gained a wide popularity due to the potential of discovering active compounds. The antioxidant properties contained in plants have been proposed as one of the mechanisms for the observed beneficial effect. Therefore, the present study investigated the antioxidant activity and total phenolic contents of various solvent extracts of Albizia procera leaves.


Antioxidant activity of the methanol extract and its derived fractions petroleum ether (APP), carbon tetrachloride (APC), dichloromethane (APD), ethyl acetate (APE), and residual aqueous fraction (APA) of the leaves of Albizia procera was performed by in vitro chemical analyses. Total phenolic content of the APM and other five fractions were also determined. APM and its derived fractions were also subjected to preliminary phytochemical screening test for various constituents.


Phytochemical screening revealed the presence of saponins, steroids, tannins, glycosides and flavonoids in the extracts. Amongst the extracts, APE showed the highest total phenolic content (449.18 ± 18.41mg of gallic acid equivalent/g of extract). In DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging test, the IC50 value of APM, APP, APC, APD, APE and APA was 43.43, 63.60, 166.18, 41.15, 11.79, and 63.06 μg/mL, respectively. Therefore, among the APM and its derived fractions, APE showed the highest antioxidant activity which is comparable to that of standard ascorbic acid (AA) (IC50 10.12 μg/mL). The total antioxidant capacity was found to be varied in different fractions. The reducing activity on ferrous ion was ranked as APE > APD > APM > APA > APC.


The above evidences suggest that APE of A. procera leaf is a potential source of natural antioxidant and can be used to prevent diseases associated with free radicals.

Antioxidants; Free radical scavenging; Phytochemical constituents; Total phenolic content