Lung tissue caspase-3 immunostaining. Lung fragments from a Wistar rat were incubated at 37°C in 50 mL MEM continuously gassed with 95% O2:5% CO2 (Panels B–C) or kept without gassing (Panels E–F). The tissues were then stained with rabbit anti-cleaved caspase-3 antibody at 0, 3, and 6 h. For Panel A–D, the lung specimen was fixed immediately after tissue collection. Please note that the H&E (Figure 6) and caspase-3 immunostain (Figure 7) pictures correspond to the same areas. There was an increase in percentage of cells in apoptosis with time (brown staining cells) in both experimental conditions. (Immunoperoxidase, 40x).
Alsuwaidi et al. BMC Research Notes 2013 6:12 doi:10.1186/1756-0500-6-12