Lung tissue histology. Lung fragments from a Wistar rat were incubated at 37°C in 50 mL MEM continuously gassed with 95% O2:5% CO2 (Panels B–C) or kept without gassing (Panels E–F). The tissues were then processed for H&E staining at 0, 3, and 6 h. For Panel A–D, the lung specimen was fixed immediately after tissue collection. The pulmonary parenchyma was better preserved in oxygenated sample over 6 h compared to unoxygenated sample, which demonstrated signs of tissue death overtime characterized by collapse of alveolar walls with sloughing of bronchiolar epithelium, loss of pneumatocytes, extravasation of red blood cells and serum fluid into alveolar spaces. (Hematoxylin and eosin, H&E, 40x).
Alsuwaidi et al. BMC Research Notes 2013 6:12 doi:10.1186/1756-0500-6-12