C57Bl/6 mouse lung tissue respiration and caspase activity. Panels A–B, in 2 independent experiments, lung fragments were incubated at 37°C in 50 mL KH buffer gassed for 60 sec with 95% O2 : 5% CO2 every h. At indicated time periods, samples were removed from the incubation medium and processed for O2 consumption and caspase activity. The values of kc (Panel A) and AMC peak area (Panel B) are plotted as a function of incubation time. Panel C–D, respiration of lung fragments that were incubated at 37°C in 50 mL MEM continuously gassed for 60 sec with 95% O2:5% CO2.
Alsuwaidi et al. BMC Research Notes 2013 6:12 doi:10.1186/1756-0500-6-12