Table 3

All bacteria real-time PCR detection with a universal system in 15 non-diarrheal stool specimens diluted in 1:5 in sterile phosphate buffer, before and after lyophilization (Ct value)
Sample n° 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15
Without lyophilization 33.01 22.22 27.99 NA NA 22.76 42.97 31.76 27.71 27.82 28.18 38.91 35.02 32.25 30.5
With lyophilization 15.61 24.24 21.70 17.41 25.19 16.78 22.02 25.94 20.58 21.47 21.24 27.43 37.34 33.91 24.28

NA, not amplified.

Donatin and Drancourt

Donatin and Drancourt BMC Research Notes 2012 5:702   doi:10.1186/1756-0500-5-702

Open Data