Table 1

Selected reference genes for analysis of expression stability
Gene symbol Gene function Primer sequence (5’ → 3’)a or SABiosciences qPCR assay IDb Amplicon length (bp)
ActB Cytoskeletal structural protein F: TGT CAC CAA CTG GGA CGA TA 165
R: GGG GTG TTG AAG GTC TCA AA
CycA Serine-threonine phosphatase inhibitor F: TAT CTG CAC TGC CAA GAC TGA GTG 126
R: CTT CTT GCT GGT CTT GCC ATT CC
18S rRNA Ribosomal subunit F: ACG GAC CAG AGC GAA AGC AT 310
R: TGT CAA TCC TGT CCG TGT CC
Rpl13A Structural component of 60S ribosomal subunit F: GGA TCC CTC CAC CCT ATG ACA 132
R: CTG GTA CTT CCA CCC GAC CTC
Tbp General transcription factor F: TGG GAT TGT ACC ACA GCT CCA 131
R: CTC ATG ATG ACT GCA GCA AAC C
GusB Exoglycosidase in lysosomes PPR43194Bb 137
Arbp Catalysis of protein synthesis PPR42394Ab 92
Cnr1 Endocannabinoid signalling PPR52793Ab 156

ActB, beta-actin; CycA, CyclophilinA; 18S rRNA, 18S subunit ribosomal RNA; Rpl13A, Ribosomal protein L13A; Tbp, TATA box binding protein; GusB, beta-glucuronidase; Arbp, Acidic ribosomal phosphoprotein P0; Cnr1, Cannabinoid Type 1 (CB1) receptor. F, forward primer; R, reverse primer.

a primer sequences are based on literature [24].

b ID, identification.

Swijsen et al.

Swijsen et al. BMC Research Notes 2012 5:685   doi:10.1186/1756-0500-5-685

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