Table 2

DNA concentration ng/μL, presented as median (maximum – minimum) measured using three different measurement methods
Extraction method UV spectroscopy qPCR Fluorometry
Reference 524.4 (1502.2 − <LOQ*) 144.2 (487.7 − 0.02) 84.2 (460.0 − <LOQ)
PrecipB 61.1 (178.3 − 3.8) 101.5 (149.4 − 0.00) 51.8 (178.4 − 3.8)
ChemLysis 16.5 (110.2 − <LOQ) 2.1 (47.7 − 0.19) 2.2 (55.2 − <LOQ)
PrecipG 14.9 (44.4 − <LOQ) 8.4 (94.5 − 0.17) 6.2 (29.6 − <LOQ)
PrecipS 4.0 (12.0 − <LOQ) 1.5 (6.1 − 0.01) 1.4 (9.5 − <LOQ)
MagBeads 4.3 (7.6 − <LOQ) 0.2 (1.3 − <LOQ) 0.4 (2.4 − <LOQ)

* below the method limit of quantification (LOQ).

Olson and Morrow

Olson and Morrow BMC Research Notes 2012 5:668   doi:10.1186/1756-0500-5-668

Open Data