Table 1

Extraction methods by application and processes
Methoda Intended sample type Lysis method Purification method
Reference General Mechanical - Bead Beat Phenol Chloroform
PrecipB Biofilm Mechanical - Bead Beat Precipitation and Silica Column
MagBeads General Mechanical - Bead Beat Magnetic Beads
PrecipS Soil Mechanical - Bead Beat Precipitation and Silica Column
ChemLysis Gram Negative Chemical - Enzymatic Silica Column
PrecipG General Mechanical - Bead Beat Precipitation and Silica Column

a DNA extraction kits are represented based on the fundamental methods utilized by each kit rather than kit names. Kits included MoBio PowerBiofilm (precipB), MoBio PowerSoil (precipS), MoBio UltraClean (precipG), Qiagen DNAeasy with Gram Negative lysis protocol (chemLysis), and Idaho Technologies Platinum Path (magBeads). Certain commercial equipment, instruments, or materials are identified in this paper in order to specify the experimental procedure adequately. Such identification is not intended to imply recommendations or endorsement by NIST, nor is it intended to imply that the materials or equipment identified are necessarily the best available for the purpose.

Olson and Morrow

Olson and Morrow BMC Research Notes 2012 5:668   doi:10.1186/1756-0500-5-668

Open Data