Expression analysis of ARHGDIB at the mRNA and protein levels in colon cancer cell lines. (A) The expression levels of known ARHGDIB transcripts and variant 6a transcripts were analysed by qRT-PCR. (B) Protein expression was confirmed by immunoblotting with the ARHGDIB-specific monoclonal antibody. Immunoblotting of tubulin, α 4a (TUBA4A) protein, was conducted as a positive control experiment. The colon cancer cell lines Colo741, DLD-1, and SW948 were examined as representative cancer cell lines whose expression profiles of variant 6a differs from that of the normal transcript. Data are expressed as mean ± SD values calculated from information obtained from an experiment repeated 4 times. Statistical significance (*p < 0.05 and **p < 0.01) was evaluated by Student’s t-test.
Hatakeyama et al. BMC Research Notes 2012 5:666 doi:10.1186/1756-0500-5-666