Table 4

Intercycler portability of the different assays on the different thermal cyclers, by comparison of the limits of reliable quantification, i.e. the most diluted standard that could be used to calculate the standard curve, expressed here as number of cells present in the most diluted standard reaction mixture
Assay ABI 7300 (2.5/22.5)a iQ5 (2.5/22.5) LC 480 (1/9) LC 480 (2/8)
Species Reference
Aggregatibacter actinomycetemcomitans Maeda et al. [35] 26 26 10 2
Fusobacterium spp. Martin et al. [37] 4 4 2 3
Parvimonas micros Bartz et al. [39] 2 1 1 2
Porphyromonas gingivalis Hyvarinen et al. [33] 9 90 36 7
Prevotella intermedia Maeda et al. [35] 11 11 4 9
Streptococcus mutans Yoshida et al. [36] 37 37 15 3
Tannerella forsythia Shelburne et al. [40] 25 25 10 2
Treponema denticola Hyvarinen et al. [33] 150 15 6 1

Legend

a: Volume of DNA extract (μl)/Volume of total mixture (μl).

Decat et al.

Decat et al. BMC Research Notes 2012 5:664   doi:10.1186/1756-0500-5-664

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