Table 3

Primers used for specificity testing, after selection based on amplification efficiency, quantification limit, and intra-run reproducibility (data not presented)
Species (reference) Correlation standard curve Amplification efficiency (%) Quantification limit (number of bacteria per 25 μl reaction)
Aggregatibacter actinomycetemcomitans[35] 0.99 89 25
Fusobacterium spp. [37] 0.99 94 4
Parvimonas micros[39] 0.99 91 2
Porphyromonas gingivalis[33] 0.99 95 9
Prevotella intermedia[35] 0.99 91 11
Treponema denticola[33] 0.99 95 150
Tannerella forsythia[40] 0.99 93 25
Streptococcus mutans[36] 0.98 115 37

Decat et al.

Decat et al. BMC Research Notes 2012 5:664   doi:10.1186/1756-0500-5-664

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