Confocal images of M. truncatula raz and WT nodules at 7, 14, 21, and 28 dpi. Images at 7 dpi were obtained with a Leica TCS SP5 Inverted Laser Scanning Confocal Microscope (Leica Microsystems, Exton, Pa.). Images at 14, 21, and 28 dpi were collected using a Leica SP1 Inverted Confocal Microscope (Leica Microsystems, Exton, Pa.). Green bars are 150 μm and red asterisks represent the stele of the root. SYTO 13, a green fluorescent nucleic acid specific stain was used to evaluate nodule development by tracking bacterial occupancy throughout the various transformations taking place during nodule formation. Some images are montages.
Lewis et al. BMC Research Notes 2012 5:657 doi:10.1186/1756-0500-5-657