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Open Access Highly Accessed Research article

Colostrum supplementation protects against exercise - induced oxidative stress in skeletal muscle in mice

Mahenderan Appukutty1, Ammu Kutty Radhakrishnan2, Kalavathy Ramasamy3, Rajesh Ramasamy4, Abu Bakar Abdul Majeed3, Mohd Ismail Noor5, Nik Shanita Safii6, Poh Bee Koon6, Karuthan Chinna7 and Nagaraja Haleagrahara8*

Author Affiliations

1 Faculty of Sports Science & Recreation, University Technology Mara, Shah Alam 40450, Kuala Lumpur, Malaysia

2 Faculty of Medicine and Health, International Medical University, Bukit Jalil 57000, Kuala Lumpur, Malaysia

3 Faculty of Pharmacy, University Technology Mara, Shah Alam 40450, Kuala Lumpur, Malaysia

4 Faculty of Medicine and Health Sciences, University Putra Malaysia, Serdang 43400, Kuala Lumpur, Malaysia

5 Faculty of Health Sciences, University Technology Mara, Shah Alam 40450, Kuala Lumpur, Malaysia

6 Faculty of Health Sciences, Universiti Kebangsaan Malaysia, Bangi 43600, Kuala Lumpur, Malaysia

7 Faculty of Medicine, University Malaya 50603, Kuala Lumpur, Malaysia

8 Nagaraja Haleagrahara, School of Veterinary and Biomedical Sciences, Faculty of Medicine, Health and Molecular Sciences, James Cook University, Townsville 4811, QLD, Australia

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BMC Research Notes 2012, 5:649  doi:10.1186/1756-0500-5-649

Published: 22 November 2012



This study examined the effects of bovine colostrum on exercise –induced modulation of antioxidant parameters in skeletal muscle in mice. Adult male BALB/c mice were randomly divided into four groups (control, colostrum alone, exercise and exercise with colostrum) and each group had three subgroups (day 0, 21 and 42). Colostrum groups of mice were given a daily oral supplement of 50 mg/kg body weight of bovine colostrum and the exercise group of mice were made to exercise on the treadmill for 30 minutes per day. Total antioxidants, lipid hydroperoxides, xanthine oxidase and super oxide dismutase level was assayed from the homogenate of hind limb skeletal muscle.


Exercise—induced a significant oxidative stress in skeletal muscles as evidenced by the elevated lipid hydroperoxides and xanthine oxidase levels. There was a significant decrease in skeletal muscle total antioxidants and superoxide dismutase levels. Daily colostrum supplement significantly reduced the lipid hydroperoxides and xanthine oxidase enzyme level and increased the total antioxidant levels in the leg muscle.


Thus, the findings of this study showed that daily bovine colostrum supplementation was beneficial to skeletal muscle to reduce the oxidant-induced damage during muscular exercise.

Bovine colostrum; Exercise; Skeletal muscle; Antioxidants; Lipid hydroperoxides