Additional file 1.

Figure S1A. G-band karyotype from the cell line retrieved from the double-hit cell lymphoma. 47,XY,der(8)t(8;12)(q24.21;p12.1)x2,+12,der(12)t(8;12)(q24.21; p12.1)x2,t(14;18)(q32;q21),der(20)t(17;20)(q23;q13) chromosomes were harvested using a routine bone marrow protocol with one hour colcemide treatment. The slides were subsequently stained using standard Trypsin-Giemsa and karyotyped using the Cytovision software. S1B-D, FISH hybridization of chromosomes retrieved from the double-hit cell lymfoma. Chromosomes were harvested as above and hybridized with the MYC BA probe, the IGH-BCL2 DF probe (Abbott) and the WCP17 and WCP20 (Metasystems), according to the manufacturer’s instructions. The pictures were retrieved using a Zeiss microscope and analyzed using the ISIS software.

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Hooper et al. BMC Research Notes 2012 5:648   doi:10.1186/1756-0500-5-648