Open Access Short Report

Sequence based analysis of U-2973, a cell line established from a double-hit B-cell lymphoma with concurrent MYC and BCL2 rearrangements

Sean D Hooper12, Xiang Jiao1, Elisabeth Sundström1, Farah L Rehman2, Christian Tellgren-Roth1, Tobias Sjöblom1 and Lucia Cavelier1*

Author Affiliations

1 Department of Immunology, Genetics and Pathology, Rudbeck Laboratory, Uppsala University, Uppsala, 751 85, Sweden

2 The Breakthrough Breast Cancer Research Centre, Institute of Cancer Research, Mary-Jean Mitchell Green Building, Chester Beatty Laboratories, Fulham Road, London, SW3 6JB, UK

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BMC Research Notes 2012, 5:648  doi:10.1186/1756-0500-5-648

Published: 22 November 2012

Additional files

Additional file 1:

Figure S1A. G-band karyotype from the cell line retrieved from the double-hit cell lymphoma. 47,XY,der(8)t(8;12)(q24.21;p12.1)x2,+12,der(12)t(8;12)(q24.21; p12.1)x2,t(14;18)(q32;q21),der(20)t(17;20)(q23;q13) chromosomes were harvested using a routine bone marrow protocol with one hour colcemide treatment. The slides were subsequently stained using standard Trypsin-Giemsa and karyotyped using the Cytovision software. S1B-D, FISH hybridization of chromosomes retrieved from the double-hit cell lymfoma. Chromosomes were harvested as above and hybridized with the MYC BA probe, the IGH-BCL2 DF probe (Abbott) and the WCP17 and WCP20 (Metasystems), according to the manufacturer’s instructions. The pictures were retrieved using a Zeiss microscope and analyzed using the ISIS software.

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Additional file 2:

Figure S2. Multicolor FISH and WCP hybridizations. S2A. M-FISH hybridization showing the t(8;12), t(14;18) and the t(17;20) translocations. S2B. WCP of chromosome 8 and 12, showing the two derived chromosomes 8, the two derived chromosomes 12 and a normal chromosome 12.

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Additional file 3:

Figure S3. Detail of an insertion of extragenous DNA and deletion of material on chromosome 20. This rearrangement was predicted by sequencing and undetected by conventional methods, but yet confirmed by PCR.

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