Screening of T. atroviridec transformants for the presence of the nptII gene. Genomic DNA was isolated from geneticin-resistant transformants and screened by PCR for the presence of the nptII gene using primers pII99Fw and pII99Rev (Table 1) which yielded a specific amplicon of 888-bp. The ~600-bp band amplified from transformant 1 indicates the presence of an additional incomplete nptII gene copy. M, DNA size standard; -, wild-type; 1–8, transformants; +, plasmid pII99.
Gruber et al. BMC Research Notes 2012 5:641 doi:10.1186/1756-0500-5-641