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Open Access Highly Accessed Research article

Generation of Trichoderma atroviride mutants with constitutively activated G protein signaling by using geneticin resistance as selection marker

Sabine Gruber1, Markus Omann12, Carolina Escobar Rodrìguez1, Theresa Radebner1 and Susanne Zeilinger1*

Author Affiliations

1 Research Area Molecular Biotechnology and Microbiology, Institute of Chemical Engineering, Vienna University of Technology, Gumpendorferstrasse 1a, Wien, Austria

2 Current address: Zuckerforschung Tulln GmbH, Josef-Reiter-Strasse 21-23, Tulln, Austria

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BMC Research Notes 2012, 5:641  doi:10.1186/1756-0500-5-641

Published: 17 November 2012

Additional files

Additional file 1:

Phenotype of nptII / tga3Q207L co-transformants on PDA and PDA + 80μg/ml geneticin. The figure shows the colony morphology of co-transformants 3/3 and 4/5 (with ectopic integration of the tga3Q207L gene) and co-transformant 2/1 (with homologous integration of the tga3Q207L gene) grown on PDA and PDA + 80μg/ml geneticin for 4 days at 28°C in the dark.

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